Assessment of collagen content in fish skin - development of a flow analysis method for hydroxyproline determination

This work describes the development of a flow injection method to determine hydroxyproline (HYP), one of collagen's most abundant amino acids. Collagen is a protein with several applications and high nutritional value. Evaluating the feasibility of using collagen from fish skin over its mammali...

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Bibliographic Details
Published in:Analytical methods 2023-11, Vol.15 (43), p.591-598
Main Authors: Melo, Maria M. P, Mesquita, Raquel B. R, Coscueta, Ezequiel R, Pintado, Manuela E, Rangel, António O. S. S
Format: Article
Language:English
Subjects:
Amino acids
Collagen
Error analysis
Fish skins
Hydroxyproline
Nutritive value
Oxidation
Skin
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Summary:This work describes the development of a flow injection method to determine hydroxyproline (HYP), one of collagen's most abundant amino acids. Collagen is a protein with several applications and high nutritional value. Evaluating the feasibility of using collagen from fish skin over its mammalian source is essential. The determination of HYP requires the pre-treatment and hydrolysis of the fish skin to break down collagen into its amino acids, and the HYP value quantified relates to the collagen content. The determination was based on the HYP oxidation with permanganate in an alkaline medium and the consequent decrease of colour intensity registered. Under optimal conditions, the developed method enables the determination of the HYP within the dynamic range of 23.8 to 500 mg L −1 , with a limit of detection (LOD) of 2.6 mg L −1 and a limit of quantification (LOQ) of 23.8 mg L −1 . Different samples were processed, and the digests were analysed by the proposed method and with the conventional procedure with good correlation (relative error < 7%). Moreover, the analyte quantification is performed faster, simpler, and more accurately, with less toxic solutions. The reproducibility of the developed method was also evaluated by calculating the relative standard deviation of the calibration curve slope (RSD < 1%). Flow injection method for the quantification of hydroxyproline to corelate with collagen content in blue shark skin.
ISSN:1759-9660
1759-9679
DOI:10.1039/d3ay01589k