Do polystyrene nanoplastics aggravate the toxicity of single contaminants (okadaic acid)? Using AGS cells as a biological model

The coexistence of nanoplastics (NPs) and various pollutants in the marine environment has become a problem that cannot be ignored. NPs and marine algae toxins are found in marine organisms and both can enter the human body through the food chain. However, the joint toxic effects of marine algae tox...

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Bibliographic Details
Published in:Environmental science. Nano 2021-11, Vol.8 (11), p.3186-321
Main Authors: Lin, Peichun, Lu, Zifan, Zhang, Yuanyuan, Liao, Xiuchun, He, Lei, Guo, Yitao, Zhou, Chunxia, Qian, Zhong-Ji, Hong, Pengzhi, Liang, Yan-Qiu, Ren, Lei, Sun, Shengli, Li, Chengyong
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
Adenocarcinoma
AKT protein
Algae
Anaemia
Anemia
Biological models (mathematics)
c-Fos protein
Calcium
Calcium (intracellular)
Caspase-3
Caspase-9
Cells
Contaminants
Cytotoxicity
Depolarization
Exposure
Fanconi syndrome
Food chains
Gene expression
Interleukin 1
Interleukin 10
Interleukin 8
MAP kinase
Marine environment
Marine organisms
Marine pollution
Membrane potential
Mitochondria
Network analysis
Okadaic acid
Plastic pollution
Pollutants
Polystyrene resins
Signal transduction
Signaling
Toxicity
Toxins
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Summary:The coexistence of nanoplastics (NPs) and various pollutants in the marine environment has become a problem that cannot be ignored. NPs and marine algae toxins are found in marine organisms and both can enter the human body through the food chain. However, the joint toxic effects of marine algae toxins and NPs on human health remain unknown. In this study, the joint toxic effects and mechanisms of polystyrene (PS) NPs and okadaic acid (OA) were investigated on human gastric adenocarcinoma (AGS) cells. AGS cells were exposed to 20 nm PS (0.5, 8 μg mL −1 ) or/and OA (5, 10 ng mL −1 ), and their cytotoxicity was assessed by measuring relevant indicators, transcriptomics, and weighted gene co-expression network analysis (WGCNA). Our data indicated that the joint toxicity of PS and OA to AGS cells was mainly characterized by a decrease in cell viability, depolarization of mitochondrial membrane potential, and a decrease in IL10 and p53 protein activity, accompanied by an increase in intracellular ROS production and calcium and IL8 levels in comparison with single contaminants. In addition, co-exposure to PS and OA caused cellular damage by activating PI3K/AKT, ERK/c-FOS and caspase-3/caspase-9 signaling pathways. Moreover, the high concentration of PS significantly enhanced the toxicity of OA. WGCNA highlighted enrichment in the Fanconi anemia pathway and MAPK signaling pathway and identified that IER3 was the hub gene in PS and OA co-exposed AGS cells. The results of this study provided insights into the joint toxicity evaluation of NPs and marine algae toxins. PS and OA jointly affected cell growth and death and inhibited DNA replication and repair by increasing ROS production, resulting in DNA damage and inducing Fanconi anemia. PS aggravates the toxicity of OA to AGS cells.
ISSN:2051-8153
2051-8161
DOI:10.1039/d1en00688f