G-quadruplex DNA inhibits unwinding activity but promotes liquid-liquid phase separation by the DEAD-box helicase Ded1p

G-quadruplex DNA interacts with the N-terminal intrinsically disordered domain of the DEAD-box helicase Ded1p, diminishing RNA unwinding activity but enhancing liquid-liquid phase separation of Ded1p in vitro and in cells. The data highlight multifaceted effects of quadruplex DNA on an enzyme with i...

Full description

Saved in:
Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2021-07, Vol.57 (6), p.7445-7448
Main Authors: Gao, Jun, Gao, Zhaofeng, Putnam, Andrea A, Byrd, Alicia K, Venus, Sarah L, Marecki, John C, Edwards, Andrea D, Lowe, Haley M, Jankowsky, Eckhard, Raney, Kevin D
Format: Article
Language:English
Subjects:
Chemistry
Cytoplasm - chemistry
Cytoplasm - metabolism
DEAD-box RNA Helicases - chemistry
DEAD-box RNA Helicases - metabolism
Deoxyribonucleic acid
DNA
DNA - genetics
DNA - metabolism
G-Quadruplexes
Intrinsically Disordered Proteins - chemistry
Intrinsically Disordered Proteins - metabolism
Liquid phases
Phase separation
Phase Transition
Protein Domains
RNA - metabolism
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae Proteins - chemistry
Saccharomyces cerevisiae Proteins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:G-quadruplex DNA interacts with the N-terminal intrinsically disordered domain of the DEAD-box helicase Ded1p, diminishing RNA unwinding activity but enhancing liquid-liquid phase separation of Ded1p in vitro and in cells. The data highlight multifaceted effects of quadruplex DNA on an enzyme with intrinsically disordered domains. G-quadruplex (G4) DNA inhibits RNA unwinding activity but promotes liquid-liquid phase separation of the DEAD-box helicase Ded1p in vitro and in cells. This highlights multifaceted effects of G4DNA on an enzyme with intrinsically disordered domains.
ISSN:1359-7345
1364-548X
DOI:10.1039/d1cc01479j