A novel 3D indirect co-culture system based on a collagen hydrogel scaffold for enhancing the osteogenesis of stem cells

In this study, the paracrine effect between adipose-derived mesenchymal stem cells (ADSCs) and osteoblasts was investigated in collagen-based three-dimensional (3D) scaffolds. 3D encapsulation of mesenchymal stem cells in hydrogel scaffolds was conducted for bone tissue regeneration. Osteoblasts wer...

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Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2020-10, Vol.8 (41), p.9481-9491
Main Authors: Kim, Hyerim, Han, Shi Huan, Kook, Yun-Min, Lee, Kyung-Mee, Jin, Yuan-Zhe, Koh, Won-Gun, Lee, Jae Hyup, Lee, Kangwon
Format: Article
Language:English
Subjects:
Alginates
Alginates - chemistry
Alginic acid
Animals
Biomedical materials
Bone growth
Cell culture
Cell Differentiation
Cell Line
Cell Proliferation
Cells, Immobilized - cytology
Coculture Techniques - methods
Collagen
Collagen - chemistry
Encapsulation
Extrusion
Humans
Hydrogels
Hydrogels - chemistry
Male
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells - cytology
Mesenchyme
Microspheres
Monoculture
Nanoparticles
Osteoblasts
Osteoblasts - cytology
Osteogenesis
Paracrine signalling
Rats, Sprague-Dawley
Regeneration
Regeneration (physiology)
Scaffolds
Stem cell transplantation
Stem cells
Tissue engineering
Tissue Scaffolds - chemistry
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Summary:In this study, the paracrine effect between adipose-derived mesenchymal stem cells (ADSCs) and osteoblasts was investigated in collagen-based three-dimensional (3D) scaffolds. 3D encapsulation of mesenchymal stem cells in hydrogel scaffolds was conducted for bone tissue regeneration. Osteoblasts were encapsulated in alginate microbeads with uniform size, which could be controlled by varying the supply voltage using electrostatic droplet extrusion. Osteoblast-encapsulated microbeads were embedded with ADSCs in collagen bulk hydrogel scaffolds with a high survival rate. The separated space between the two types of cells made it possible to confirm ADSC differentiation into osteogenic lineages in the 3D collagen hydrogel scaffold by the paracrine effect in vitro . Furthermore, co-cultured ADSC and osteoblasts showed enhanced bone formation compared with the ADSC monoculture group in the rat calvarial defect model. The system developed in this study provides a novel in vitro tissue model for bone regeneration without exogenous factors, and it has the potential to be used to study the paracrine effect in various co-culture systems in the near future. In this study, the paracrine effect between adipose-derived mesenchymal stem cells (ADSCs) and osteoblasts was investigated in collagen-based three-dimensional (3D) scaffolds.
ISSN:2050-750X
2050-7518
DOI:10.1039/d0tb01770a