Impaired IL‐1 β ‐induced neutrophil accumulation in tachykinin NK 1 receptor knockout mice

Tachykinin NK 1 receptors play an important role in the development of neurogenic inflammatory responses. We have used the murine air‐pouch model to investigate whether the neurogenic component of the cellular inflammatory response to interleukin‐1β (IL‐1β, 10 ng into the air‐pouch) is altered in NK...

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Published in:British journal of pharmacology 2009-02, Vol.124 (6), p.1013-1015
Main Authors: Ahluwalia, Amrita, De Felipe, Carmen, O'Brien, John, Hunt, Stephen P., Perretti, Mauro
Format: Article
Language:English
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Summary:Tachykinin NK 1 receptors play an important role in the development of neurogenic inflammatory responses. We have used the murine air‐pouch model to investigate whether the neurogenic component of the cellular inflammatory response to interleukin‐1β (IL‐1β, 10 ng into the air‐pouch) is altered in NK 1 receptor knockout mice compared to wild type controls. Air‐pouches were washed following a 4 h IL‐1β treatment, the wash collected and neutrophil number estimated using a Neubauer haemocytometer. The response to IL‐1β was significantly attenuated in NK 1 receptor +/− (40% reduction) and −/− mice (62% reduction) compared to wild type controls (+/+), whilst the response to cytokine‐induced neutrophil chemoattractant (CINC, 0.3 μg) was unaffected. The response to substance P (7.5 nmol) was attenuated by approximately 50% in both NK 1 receptor +/− and −/− mice compared to wild type controls. In conclusion NK 1 receptors play a significant role in the cellular response to IL‐1β in a model of inflammation. British Journal of Pharmacology (1998) 124 , 1013–1015; doi: 10.1038/sj.bjp.0701978
ISSN:0007-1188
1476-5381
DOI:10.1038/sj.bjp.0701978