Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus

We have obtained fertile transgenic wheat plants resistant to the broad spectrum he bicide Basta(R) (active ingredient phos phinothricin, PPT) by high velocity microprojectile bombardment. The plasmid pBARGUS was used to deliver the selectable bar gene into cells of Type C long-term regenerable embr...

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Bibliographic Details
Published in:Bio/technology (New York, N.Y. 1983) N.Y. 1983), 1992-06, Vol.10 (6), p.667-674
Main Authors: Vasil, V. (University of Florida, Gainesville, FL), Castillo, A.M, Fromm, M.E, Vasil, I.K
Format: Article
Language:English
Subjects:
ACILTRANSFERASA
ACYLTRANSFERASE
ACYLTRANSFERASES
Agronomy. Soil science and plant productions
bar gene
basta
Biological and medical sciences
Biotechnology
Biotechnology & Applied Microbiology
CAL
CALLO
CALLUS
CHEMICAL RESISTANCE
DESARROLLO EMBRIONARIO
DEVELOPPEMENT EMBRYONNAIRE
DIRECT DNA UPTAKE
EMBRYONIC DEVELOPMENT
Fundamental and applied biological sciences. Psychology
GENE
GENE TRANSFER
GENES
Genetic engineering
Genetic technics
GENETIC TRANSFORMATION
GLUFOSINATE
HERBICIDAS
HERBICIDE
HERBICIDES
Life Sciences & Biomedicine
Methods. Procedures. Technologies
phosphinothricin acetyltransferase
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
PLASMIDE
PLASMIDIOS
PLASMIDS
REGENERACION
REGENERATION
REGENERATIVE ABILITY
REPORTER GENES
RESISTANCE AUX PRODUITS CHIMIQUES
RESISTENCIA QUIMICA
Science & Technology
STRUCTURAL GENES
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Transgenic animals and transgenic plants
TRANSGENIC PLANTS
TRITICUM AESTIVUM
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Summary:We have obtained fertile transgenic wheat plants resistant to the broad spectrum he bicide Basta(R) (active ingredient phos phinothricin, PPT) by high velocity microprojectile bombardment. The plasmid pBARGUS was used to deliver the selectable bar gene into cells of Type C long-term regenerable embryogenic callus. Phosphinothricin acetyltransferase (PAT) enzyme activity encoded by the bar gene was demonstrated in four independent putative transformed callus lines selected on Basta(R) from two cultivars. Although somatic embryos and shoots were formed in each of the four lines, plants were recovered only from two. More than 100 green R0 plants were regenerated from the first callus line, of which 40 were grown to maturity. PAT activity was shown in each of the 28 R0 plants tested. Southern analyses confirmed the presence of the bar gene in all of the callus fines, and in each of the R0 and two of the four R1 plants tested. Transformed R0, R1 and R2 plants were resistant to topical applications of Basta(R), and the bar gene segregated as a dominant Mendelian trait in R1 and R2 plants.
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt0692-667