Flow cytometric characteristics of subchloroplast particles prepared by the action of various detergents

Agranal thylakoid membranes from leaves of Phaseolus vulgaris were fragmented using seven distinct detergents: digitonin, Triton X-100, cetylpyridinium chloride, sodium dodecylsulfate, and Zwittergents 3-12, 3-14, and 3-16, differing in chemical composition and/or electric charges. Subchloroplast pa...

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Bibliographic Details
Published in:Photosynthetica 1998-01, Vol.34 (3), p.447-464
Main Authors: Wilhelmova, N. (Akademie Ved, Prague (Czech Republic). Ustav Experimentalni Botaniky), Vanek, S, Wilhelm, J
Format: Article
Language:English
Subjects:
ANALYTICAL METHODS
CHLORIDES
CHLOROPLASTE
CHLOROPLASTS
CHLORURE
CLOROPLASTO
CLORUROS
DIMENSION
DIMENSIONS
PHASEOLUS VULGARIS
SURFACE ACTIVE AGENTS
SURFACTANT
SURFACTANTES
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
THYLAKOIDE
THYLAKOIDS
TILACOIDES
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Summary:Agranal thylakoid membranes from leaves of Phaseolus vulgaris were fragmented using seven distinct detergents: digitonin, Triton X-100, cetylpyridinium chloride, sodium dodecylsulfate, and Zwittergents 3-12, 3-14, and 3-16, differing in chemical composition and/or electric charges. Subchloroplast particles were examined by flow cytometry to determine their size and shape. Simple direct proportionality between the particle density and their size was not observed in any case, nevertheless, bigger particles were more abundant in fractions of higher density. Some vesicles had even a larger size than the original thylakoids.
ISSN:0300-3604
1573-9058
DOI:10.1023/A:1006876420980