Protective Effect of Anthocyanins from Black Soybean Seed Coats on UVB-Induced Apoptotic Cell Death in Vitro and in Vivo

UVB radiation proves to be one of the most relevant environmental risks because of its hazardous effects, such as premature skin aging and especially skin photocarcinogenesis. Anthocyanins, water-soluble pigments present in plants, are known to be powerful antioxidants that help protect plants from...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2008-11, Vol.56 (22), p.10600-10605
Main Authors: Tsoyi, Konstantin, Park, Hyung Bin, Kim, Young Min, Chung, Jong Il, Shin, Sung Chul, Shim, Hae Jung, Lee, Won Suk, Seo, Han Geuk, Lee, Jae Heun, Chang, Ki Churl, Kim, Hye Jung
Format: Article
Language:English
Subjects:
Animals
Anthocyanins
Anthocyanins - pharmacology
antioxidant activity
apoptosis
Apoptosis - drug effects
Apoptosis - radiation effects
Bax
Bcl-2
black soybeans
Caspase Inhibitors
caspase-3
Cell Line, Transformed
cell lines
Chemical Aspects of Biotechnology/Molecular Biology
cysteine proteinases
Enzyme Inhibitors - pharmacology
Female
Glycine max - chemistry
Humans
Keratinocytes
Mice
Mice, Hairless
protective effect
radioprotective effect
reactive oxygen species
Reactive Oxygen Species - antagonists & inhibitors
ROS
Seeds - chemistry
skin
Skin - cytology
soybeans
testa
topical application
ultraviolet radiation
Ultraviolet Rays - adverse effects
UVB
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Summary:UVB radiation proves to be one of the most relevant environmental risks because of its hazardous effects, such as premature skin aging and especially skin photocarcinogenesis. Anthocyanins, water-soluble pigments present in plants, are known to be powerful antioxidants that help protect plants from UV damage. In this study, we aimed at investigating the protective effect of anthocyanins from black soybean [Glycine max (L.) Merr] seed coats on UVB-induced apoptosis, and furthermore, we investigated the molecular mechanism responsible for regulation of apoptosis in vitro and in vivo. Pretreatment with anthocyanins reduced UVB-induced reactive oxygen species levels and inhibited UVB-induced apoptotic cell death through the prevention of caspase-3 pathway activation and reduction of proapoptotic Bax protein levels. UVB irradiation induced apoptotic cell death, which was inhibited by topical application of anthocyanins in hairless mice. It is concluded that anthocyanins from the seed coat of black soybeans may be useful compounds to modulate UVB-induced photoaging.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf802112c