Fumonisin Production by Fusarium verticillioides Strains Isolated from Maize in Mexico and Development of a Polymerase Chain Reaction to Detect Potential Toxigenic Strains in Grains

Fumonisins are mycotoxins produced by Fusarium verticillioides (Sacc. Nirenberg) in maize (Zea mays L.), a staple crop in Mexico. In this study, we report the isolation and identification of 67 Fusarium strains isolated from maize kernels collected in Northwest and Central Mexico. The strains were c...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2005-11, Vol.53 (22), p.8565-8571
Main Authors: Sánchez-Rangel, Diana, SanJuan-Badillo, Andrea, Plasencia, Javier
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Cereal and baking product industries
DNA, Fungal - analysis
Food Contamination - analysis
Food industries
Food toxicology
Fumonisins - metabolism
Fundamental and applied biological sciences. Psychology
Fusarium - genetics
Fusarium - isolation & purification
Fusarium - metabolism
Mexico
Molecular Sequence Data
Polymerase Chain Reaction - methods
Zea mays - microbiology
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Summary:Fumonisins are mycotoxins produced by Fusarium verticillioides (Sacc. Nirenberg) in maize (Zea mays L.), a staple crop in Mexico. In this study, we report the isolation and identification of 67 Fusarium strains isolated from maize kernels collected in Northwest and Central Mexico. The strains were characterized regarding fumonisin B1 production and the presence of the FUM1 gene. F. verticillioides was the predominant species isolated in both geographic regions, but the isolates from Northwest Mexico produced higher levels of fumonisin. A polymerase chain reaction (PCR)-based method, to detect a region of the FUM1 gene involved in fumonisin biosynthesis, was developed and employed to detect mycotoxigenic fungi in pure culture and in contaminated maize. The presence of the FUM1 gene was associated with fumonisin production in most isolates, except seven that did not synthesize fumonisin but contained the gene in their genome. The PCR method allowed the direct detection of fungal contamination in ground corn and could be employed to screen for the presence of potential mycotoxigenic fusaria. Keywords: Mycotoxin; fumonisin B1; Fusarium verticillioides; polymerase chain reaction; maize
ISSN:0021-8561
1520-5118
DOI:10.1021/jf0514827