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Stability of pectinesterases of Marsh white grapefruit pulp

Differences in thermal and storage stability, as well as susceptibility to proteolysis, were demonstrated in two forms of pectinesterase (PE) isolated from Marsh white grapefruit. It was shown previously that the two forms contain different amounts of attached carbohydrate. Partial removal of the ca...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1991-06, Vol.39 (6), p.1075-1079
Main Authors: Seymour, Thomas A, Preston, James F, Wicker, Louise, Lindsay, James A, Wei, Cheng I, Marshall, Maurice R
Format: Article
Language:English
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Summary:Differences in thermal and storage stability, as well as susceptibility to proteolysis, were demonstrated in two forms of pectinesterase (PE) isolated from Marsh white grapefruit. It was shown previously that the two forms contain different amounts of attached carbohydrate. Partial removal of the carbohydrate moiety decreased thermostability of thermostable (TS) PE. The presence of an O-glycosidic linkage was demonstrated in TS PE. The O-linked oligosaccharides have an average degree of polymerization of 2.8 and were composed of four different monosaccharides
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00006a014