Antibiofouling Polymer-Coated Superparamagnetic Iron Oxide Nanoparticles as Potential Magnetic Resonance Contrast Agents for in Vivo Cancer Imaging

We report the fabrication and characterization of antifouling polymer-coated magnetic nanoparticles as nanoprobes for magnetic resonance (MR) contrast agents. Magnetite superparamagnetic iron oxide nanoparticles (SPION) were coated with the protein- or cell-resistant polymer, poly(TMSMA-r-PEGMA), to...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2006-06, Vol.128 (22), p.7383-7389
Main Authors: Lee, Haerim, Lee, Eunhye, Kim, Do Kyung, Jang, Nam Kyu, Jeong, Yong Yeon, Jon, Sangyong
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carcinoma, Lewis Lung - diagnostic imaging
Carcinoma, Lewis Lung - pathology
Cell Line, Tumor
Cell Survival - drug effects
Coated Materials, Biocompatible - chemical synthesis
Coated Materials, Biocompatible - chemistry
Contrast Media - administration & dosage
Contrast Media - adverse effects
Contrast Media - chemistry
Contrast media. Radiopharmaceuticals
Drug Stability
Ferrosoferric Oxide - administration & dosage
Ferrosoferric Oxide - chemistry
Investigative techniques, diagnostic techniques (general aspects)
Macrophages - drug effects
Macrophages - metabolism
Magnetic Resonance Imaging
Magnetics
Materials Testing
Medical sciences
Metal Nanoparticles - administration & dosage
Metal Nanoparticles - adverse effects
Mice
Miscellaneous. Technology
Neoplasm Transplantation
Pharmacology. Drug treatments
Polymers - chemical synthesis
Polymers - chemistry
Radiodiagnosis. Nmr imagery. Nmr spectrometry
Radiography
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Summary:We report the fabrication and characterization of antifouling polymer-coated magnetic nanoparticles as nanoprobes for magnetic resonance (MR) contrast agents. Magnetite superparamagnetic iron oxide nanoparticles (SPION) were coated with the protein- or cell-resistant polymer, poly(TMSMA-r-PEGMA), to generate stable, protein-resistant MR probes. Coated magnetic nanoparticles synthesized using two different preparation methods (in situ and stepwise, respectively) were both well dispersed in PBS buffer at a variety of pH conditions (pH 1−10). In addition, dynamic light scattering data revealed that their sizes were not altered even after 24 h of incubation in 10% serum containing cell culture medium, indicative of a lack of protein adsorption on their surfaces. When the antibiofouling polymer-coated SPION were incubated with macrophage cells, uptake was significantly lower in comparison to that of the popular contrast agent, Feridex I.V., suggesting that the polymer-coated SPION can be long-circulated in plasma by escaping from uptake by the reticular endothelial system (RES) such as macrophages. Indeed, when the coated SPION were administered to tumor xenograft mice by intravenous injection, the tumor could be detected in T2-weighted MR images within 1 h as a result of the accumulation of the nanomagnets within the tumor site. Although the poly(TMSMA-r-PEGMA)-coated SPION do not have any targeting ligands on their surface, they are potentially useful for cancer diagnosis in vivo.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja061529k