Synthesis of Oligodeoxynucleotides Containing N 4-Mercaptoethylcytosine and Their Use in the Preparation of Oligonucleotide−Peptide Conjugates Carrying c-myc Tag-Sequence

The preparation and properties of oligodeoxynucleotides containing mercaptoethyl groups at position N-4 of cytosine are described. The resulting thiol-oligodeoxynucleotides were reacted with a maleimido−peptide carrying the c-myc tag-sequence. The peptide−oligonucleotide conjugate is specifically re...

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Bibliographic Details
Published in:Bioconjugate chemistry 1998-11, Vol.9 (6), p.831-837
Main Authors: Gottschling, Dirk, Seliger, Hartmut, Tarrasón, Gema, Piulats, Jaume, Eritja, Ramon
Format: Article
Language:English
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Summary:The preparation and properties of oligodeoxynucleotides containing mercaptoethyl groups at position N-4 of cytosine are described. The resulting thiol-oligodeoxynucleotides were reacted with a maleimido−peptide carrying the c-myc tag-sequence. The peptide−oligonucleotide conjugate is specifically recognized by an anti c-myc monoclonal antibody, thus constituting a labeling system with sensitivity similar to other existing methods of nonradioactive labeling.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc980074o