Molecular Modeling of Hapten Structure and Relevance to Broad Specificity Immunoassay of Sulfonamide Antibiotics

Molecular modeling of hapten structure was used to predict and influence, through appropriate synthetic work, the outcome of an immunization program. Examination of the structures of sulfonamide antibiotics led to the development of a hypothesis and the consequent synthesis of a sulfacetamide−protei...

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Bibliographic Details
Published in:Bioconjugate chemistry 1999-07, Vol.10 (4), p.583-588
Main Authors: Spinks, C. A, Wyatt, G. M, Lee, H. A, Morgan, M. R. A
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - analysis
Anti-Infective Agents, Local - chemistry
Anti-Infective Agents, Local - immunology
Antibody Specificity
Enzyme-Linked Immunosorbent Assay
Haptens - chemistry
Immunoassay
Models, Molecular
Rabbits - immunology
Serum Albumin, Bovine - chemistry
Sulfacetamide - chemistry
Sulfacetamide - immunology
Sulfachlorpyridazine - chemistry
Sulfachlorpyridazine - immunology
Sulfonamides - analysis
Sulfonamides - chemistry
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Summary:Molecular modeling of hapten structure was used to predict and influence, through appropriate synthetic work, the outcome of an immunization program. Examination of the structures of sulfonamide antibiotics led to the development of a hypothesis and the consequent synthesis of a sulfacetamide−protein immunogen aimed at the generation of broad specificity anti-sulfonamide antibodies. The antisera generated, alongside anti-sulfachlorpyradizine antisera generated at the same time, were characterized for cross-reactions against a range of sulfonamide drugs, and were found to exhibit good but not the desired broad specificity. Discussion is presented as to the reasons for the failure of the hypothesis. Further hypotheses are developed and speculation is made as to the future of molecular modeling in immunochemical research.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc980054m