Plasmonic Au@Ag@mSiO 2 Nanorattles for In Situ Imaging of Bacterial Metabolism by Surface-Enhanced Raman Scattering Spectroscopy

It is well known that microbial populations and their interactions are largely influenced by their secreted metabolites. Noninvasive and spatiotemporal monitoring and imaging of such extracellular metabolic byproducts can be correlated with biological phenotypes of interest and provide new insights...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2021-12, Vol.13 (51), p.61587-61597
Main Authors: De Marchi, Sarah, García-Lojo, Daniel, Bodelón, Gustavo, Pérez-Juste, Jorge, Pastoriza-Santos, Isabel
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Escherichia coli - isolation & purification
Escherichia coli - metabolism
Gold - chemistry
Hydrogen-Ion Concentration
Materials Testing
Particle Size
Silicon Dioxide - chemistry
Silver - chemistry
Spectrum Analysis, Raman
Surface Properties
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Summary:It is well known that microbial populations and their interactions are largely influenced by their secreted metabolites. Noninvasive and spatiotemporal monitoring and imaging of such extracellular metabolic byproducts can be correlated with biological phenotypes of interest and provide new insights into the structure and development of microbial communities. Herein, we report a surface-enhanced Raman scattering (SERS) hybrid substrate consisting of plasmonic Au@Ag@mSiO nanorattles for optophysiological monitoring of extracellular metabolism in microbial populations. A key element of the SERS substrate is the mesoporous silica shell encapsulating single plasmonic nanoparticles, which furnishes colloidal stability and molecular sieving capabilities to the engineered nanostructures, thereby realizing robust, sensitive, and reliable measurements. The reported SERS-based approach may be used as a powerful tool for deciphering the role of extracellular metabolites and physicochemical factors in microbial community dynamics and interactions.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.1c21812