The Intracellular Number of Magnetic Nanoparticles Modulates the Apoptotic Death Pathway after Magnetic Hyperthermia Treatment

Magnetic hyperthermia is a cancer treatment based on the exposure of magnetic nanoparticles to an alternating magnetic field in order to generate local heat. In this work, 3D cell culture models were prepared to observe the effect that a different number of internalized particles had on the mechanis...

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Published in:ACS applied materials & interfaces 2020-09, Vol.12 (39), p.43474-43487
Main Authors: Beola, Lilianne, Asín, Laura, Roma-Rodrigues, Catarina, Fernández-Afonso, Yilian, Fratila, Raluca M, Serantes, David, Ruta, Sergiu, Chantrell, Roy W, Fernandes, Alexandra R, Baptista, Pedro V, de la Fuente, Jesús M, Grazú, Valeria, Gutiérrez, Lucía
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Biological and Medical Applications of Materials and Interfaces
Cells, Cultured
Hyperthermia, Induced
Kinetics
Macrophages - drug effects
Magnetic Fields
Magnetite Nanoparticles - chemistry
Mice
Monte Carlo Method
Particle Size
RAW 264.7 Cells
Surface Properties
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cited_by cdi_FETCH-LOGICAL-a370t-dc58387b9adbb9f055525fc741a1094512af0fbf813a665a07ceea3f5119b89b3
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container_issue 39
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container_title ACS applied materials & interfaces
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creator Beola, Lilianne
Asín, Laura
Roma-Rodrigues, Catarina
Fernández-Afonso, Yilian
Fratila, Raluca M
Serantes, David
Ruta, Sergiu
Chantrell, Roy W
Fernandes, Alexandra R
Baptista, Pedro V
de la Fuente, Jesús M
Grazú, Valeria
Gutiérrez, Lucía
description Magnetic hyperthermia is a cancer treatment based on the exposure of magnetic nanoparticles to an alternating magnetic field in order to generate local heat. In this work, 3D cell culture models were prepared to observe the effect that a different number of internalized particles had on the mechanisms of cell death triggered upon the magnetic hyperthermia treatment. Macrophages were selected by their high capacity to uptake nanoparticles. Intracellular nanoparticle concentrations up to 7.5 pg Fe/cell were measured both by elemental analysis and magnetic characterization techniques. Cell viability after the magnetic hyperthermia treatment was decreased to
doi_str_mv 10.1021/acsami.0c12900
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At low intracellular magnetic nanoparticle amounts (below 1 pg Fe/cell), the intrinsic route was the main mechanism to induce apoptosis, as observed by the high Bax/Bcl-2 mRNA ratio and low caspase-8 activity. In contrast, at higher concentrations of internalized magnetic nanoparticles (1–7.5 pg Fe/cell), the extrinsic route was observed through the increased activity of caspase-8. Nevertheless, both mechanisms may coexist at intermediate iron concentrations. 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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Animals
Apoptosis - drug effects
Biological and Medical Applications of Materials and Interfaces
Cells, Cultured
Hyperthermia, Induced
Kinetics
Macrophages - drug effects
Magnetic Fields
Magnetite Nanoparticles - chemistry
Mice
Monte Carlo Method
Particle Size
RAW 264.7 Cells
Surface Properties
title The Intracellular Number of Magnetic Nanoparticles Modulates the Apoptotic Death Pathway after Magnetic Hyperthermia Treatment
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