Complete Bioconversion of Protopanaxadiol-Type Ginsenosides to Compound K by Extracellular Enzymes from the Isolated Strain Aspergillus tubingensis

A compound K-producing fungus was isolated from meju (fermented soybean brick) and identified as the generally recognized as safe (GRAS) strain . The extracellular enzymes obtained after the cultivation of 6 days in the medium with 20 g/L citrus pectin as an inducer showed the highest compound K-pro...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2021-01, Vol.69 (1), p.315-324
Main Authors: Kim, Se-A, Jeong, Eun-Bi, Oh, Deok-Kun
Format: Article
Language:English
Subjects:
Aspergillus - chemistry
Aspergillus - enzymology
Aspergillus - metabolism
Biotransformation
Fungal Proteins - chemistry
Fungal Proteins - metabolism
Ginsenosides - chemistry
Molecular Structure
Panax - chemistry
Plant Extracts - chemistry
Plant Extracts - metabolism
Sapogenins - chemistry
Sapogenins - metabolism
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Summary:A compound K-producing fungus was isolated from meju (fermented soybean brick) and identified as the generally recognized as safe (GRAS) strain . The extracellular enzymes obtained after the cultivation of 6 days in the medium with 20 g/L citrus pectin as an inducer showed the highest compound K-producing activity among the inducers tested. Under the optimized conditions of 0.05 mM MgSO , 55 °C, pH 4.0, 13.4 mM protopanaxadiol (PPD)-type ginsenosides, and 11 mg/mL enzymes, the extracellular enzymes from completely converted PPD-type ginsenosides in the ginseng extract to 13.4 mM (8.35 mg/mL) compound K after 20 h, with the highest concentration and productivity among the results reported so far. As far as we know, this is the first GRAS enzyme to completely convert all PPD-type ginsenosides to compound K.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.0c07424