Enantioselective Effect of Flutriafol on Growth, Deoxynivalenol Production, and TRI Gene Transcript Levels in Fusarium graminearum

In recent years, deoxynivalenol (DON) has frequently been detected in wheat grains and their products. The enantioselective impact of flutriafol on the growth and DON biosynthesis of Fusarium graminearum was investigated in relation to water activity (αw, 0.97 and 0.99) and temperature (20, 25, and...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2021-02, Vol.69 (5), p.1684-1692
Main Authors: Li, Chaofeng, Fan, Shuai, Wen, Yan, Tan, Zhenchao, Liu, Chenglan
Format: Article
Language:English
Subjects:
Food Safety and Toxicology
Fungal Proteins - genetics
Fungal Proteins - metabolism
Fungicides, Industrial - chemistry
Fungicides, Industrial - pharmacology
Fusarium - drug effects
Fusarium - genetics
Fusarium - growth & development
Fusarium - metabolism
Gene Expression Regulation, Fungal - drug effects
Plant Diseases - microbiology
Stereoisomerism
Triazoles - chemistry
Triazoles - pharmacology
Trichothecenes - metabolism
Triticum - microbiology
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Summary:In recent years, deoxynivalenol (DON) has frequently been detected in wheat grains and their products. The enantioselective impact of flutriafol on the growth and DON biosynthesis of Fusarium graminearum was investigated in relation to water activity (αw, 0.97 and 0.99) and temperature (20, 25, and 30 °C) on the wheat-based medium. R-(−)-flutriafol exhibited higher bioactivity than S-(+)-flutriafol and Rac-flutriafol under the above conditions. Flutriafol enantiomers reduced or stimulated DON biosynthesis depending on αw. DON levels were negligible after 14 or 7 days of incubation times under 0.97 and 0.99 aw, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses showed that the expression levels of trichothecene biosynthetic (TRI) genes of F. graminearum under 0.97 aw were significantly higher than those under 0.99 aw. In addition, R-(−)-flutriafol can induce more TRI gene expression than S-(+)-flutriafol. Taken together, this study indicated that aw and temperature play important roles in regulating DON biosynthesis in F. graminearum with flutriafol enantiomers.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.0c06800