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Redox imbalance accompanies loss of viability in seeds of two cacti species buried in situ
Abstract This work provides insights into the deterioration of cacti seeds of Escontria chiotilla (F.A.C. Weber ex K. Schum) and Stenocereus pruinosus (Otto ex Pfeiff.) Buxbaum stored ex situ at 25 °C, under dry and dark conditions or buried in situ conditions in a xerophytic shrubland. Viability, g...
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Published in: | Seed science research 2024-04, p.1-9 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Summary: | Abstract
This work provides insights into the deterioration of cacti seeds of
Escontria chiotilla
(F.A.C. Weber ex K. Schum) and
Stenocereus pruinosus
(Otto ex Pfeiff.) Buxbaum stored
ex situ
at 25 °C, under dry and dark conditions or buried
in situ
conditions in a xerophytic shrubland. Viability, germination speed, electrolyte leakage and indicators of the redox balance including glutathione content, glutathione half-cell reduction potential (E
GSSG/2GSH
) and malondialdehyde (MDA), oxidized protein content, together with water-soluble antioxidant enzyme activity were assessed. Over a period of two years of storage, viability was maintained when seeds were stored
ex situ
at 7–9% water content compared to seeds buried in the soil. A second burial experiment showed that seeds of
E. chiotilla
maintained viability during a year of storage that included a rainy season followed by a dry season. Thereafter, they died rapidly during the second rainy season. In contrast, those of
S. pruinosus
started to lose viability after 6 months of burial at the end of the rainy season and were mostly dead at the end of the dry season. This difference in persistence between species was related to a difference in the glutathione content and antioxidant enzyme activities. In both storage experiments, the loss of viability of both species was associated to a E
GSSG/2GSH
shift to a more oxidative state during burial. Yet, contents in MDA and oxidized soluble proteins were not related to redox imbalance and loss of viability, indicating that these compounds are not good markers of oxidative stress in cacti seeds during storage. |
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ISSN: | 0960-2585 1475-2735 |
DOI: | 10.1017/S0960258524000011 |