The plasma membrane magnesium transporter CsMGT5 mediates magnesium uptake and translocation under magnesium limitation in tea plants (Camellia sinensis L.)

•Ten CsMGT5 were comprehensively identified and analyzed.•CsMGT5 was highly expressed and induced by low-Mg2+ treatments in roots.•CsMGT5 is a high-affinity Mg2+transporter, which may mediate Mg2+ uptake and translocation under Mg2+limitation in tea plants. Magnesium transporter/Mitochondrial RNA sp...

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Published in:Scientia horticulturae 2023-02, Vol.310, p.111711, Article 111711
Main Authors: Li, Jing, Hu, Xinlong, Zhang, Ruiming, Li, Qinghui, Xu, Wenluan, Zhang, Luyu, Guo, Fei, Zhao, Hua, Wang, Pu, Wang, Yu, Ni, Dejiang, Wang, Mingle
Format: Article
Language:English
Subjects:
Camellia sinensis
CsMGT5
Magnesium homeostasis
Magnesium transporter
Transgene
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Summary:•Ten CsMGT5 were comprehensively identified and analyzed.•CsMGT5 was highly expressed and induced by low-Mg2+ treatments in roots.•CsMGT5 is a high-affinity Mg2+transporter, which may mediate Mg2+ uptake and translocation under Mg2+limitation in tea plants. Magnesium transporter/Mitochondrial RNA splicing 2 (MGT/MRS2) plays an important role in the growth and development of plants by maintaining the magnesium ion (Mg2+) homeostasis. However, MGTs gene family has not been thoroughly characterized in Camellia sinensis. In this study, ten CsMGTs were comprehensively identified and analyzed from tea plant genome databases (i.e., 'Shuchazao' and 'Yunkang 10’). Expression analyses showed that the transcription of CsMGTs was differentially regulated by various abiotic stresses (i.e., low-Mg2+, drought, and salt). Moreover, CsMGTs exhibited specific expression in different tissues, in which CsMGT5 was highly expressed in roots. Further analyses indicated that CsMGT5 was highly expressed and induced by low-Mg2+ treatments in roots of five different tea varieties, which was highly homologous to the key Mg2+ transporter AtMGT6 in Arabidopsis. Correlation analysis presented that the expression level of CsMGT5 in roots of five tea cultivars was significantly correlated with Mg2+ content in C. sinensis roots (R2 = 0.8591, P < 0.05). Functional analyses showed that CsMGT5, which was located in the plasma membrane, was a high-affinity Mg2+transporter in Salmonella, and heterologous expression of CsMGT5 conferred low-Mg2+ tolerance by prompting the accumulation of Mg2+ in roots and leaves of transgenic Arabidopsis. Overall, these results suggest that CsMGT5 mediates Mg2+ uptake and translocation under Mg2+ limitation in tea plants.
ISSN:0304-4238
1879-1018
DOI:10.1016/j.scienta.2022.111711