High efficient gene targeting in rice field eel Monopterus albus by transcription activator-like effector nucleases

Genome editing plays an important role in the research of gene functions, gene correction and cell replacement therapy. Tradition- ally, genome editing is mainly dependent on the technology of homologous recombination （HR） and embryonic stem （ES） cell lines in mammals [1]. However, genome editing is...

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Bibliographic Details
Published in:Science bulletin (Beijing) 2017-02, Vol.62 (3), p.162-164
Main Authors: Feng, Ke, Luo, Hongrui, Li, Yongming, Chen, Ji, Wang, Yaping, Sun, Yonghua, Zhu, Zuoyan, Hu, Wei
Format: Article
Language:English
Subjects:
ES细胞系
基因打靶
基因组
子样
核酸酶
胚胎干细胞
转录激活
黄鳝
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Summary:Genome editing plays an important role in the research of gene functions, gene correction and cell replacement therapy. Tradition- ally, genome editing is mainly dependent on the technology of homologous recombination （HR） and embryonic stem （ES） cell lines in mammals [1]. However, genome editing is severely limited by the low efficiency of HR and lack of ES cell lines in most organisms. Recently, the development of zinc finger nucleases （ZFNs）,
ISSN:2095-9273
DOI:10.1016/j.scib.2017.01.018