A fluorescent Salamo-Salen-Salamo-Zn(II) sensor for bioimaging and biosensing H2PO4- in Zebrafish and plants
A novel Salamo-Salen-Salamo-Zn(II) complex fluorescent sensor ZT was successfully synthesized, and exhibited fluorescent quenching effect and excellent applicability through solid-state fluorescent strips and biological imaging experiments on zebrafish and bean sprout to highly sensitive monitoring...
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| Published in: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2023-12, Vol.303, p.123159, Article 123159 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | eng |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | A novel Salamo-Salen-Salamo-Zn(II) complex fluorescent sensor ZT was successfully synthesized, and exhibited fluorescent quenching effect and excellent applicability through solid-state fluorescent strips and biological imaging experiments on zebrafish and bean sprout to highly sensitive monitoring H2PO4- ions.
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•A novel and effective Salamo-Salen-Salamo-Zn fluorescent sensor ZT was successfully developed.•It is worth noting that ZT serves as a fluorescence ON-OFF sensor by adding H2PO4- in the presence of excess other anions.•The sensor ZT was successfully studied using the solid-state fluorescence of the test strips.•Bioimaging experiments were conducted on zebrafish and bean sprouts using a confocal microscope.
A newly designed and synthesized Salamo-Salen-Salamo-Zn(II) complex sensor (sensor ZT) was extensively explored for anion sensing studies. The selectivity and sensitivity of the sensor ZT towards H2PO4- ions were based on ICT and CHEF effects, and via displacement pathways in DMSO/H2O (9:1, v/v) medium in the presence of other anions like, PO43-, HPO42- and P2O74- in a short time, separately. The prepared ZT sensor has excellent association constant and low detection lines. The sensing mechanism and binding mode of the sensor were studied by UV–Vis spectroscopy, HR-MS, 1H NMR titration and theory calculations (DFT & TD-DFT) for analytes. The time response and stability of the sensor are also given. Meanwhile, the sensor ZT can be widely used as a simple and effective solid-state optical sensor to detect H2PO4- by intuitive fluorescence changes. In addition, besides the environment can be used as a powerful instrument for detecting H2PO4-, based on the good biocompatibility and tissue permeability of ZT, effectively monitoring H2PO4- in cellular distribution by confocal microscopy using Zebrafish and bean sprout. |
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| ISSN: | 1386-1425 |