On-line flow injection analysis using gold particle modified carbon electrode amperometric detection for real-time determination of glucose in immobilized enzyme hydrolysate of waste bamboo chopsticks
[Display omitted] ► A novel gold particle modified glucose sensor carbon electrode is fabricated. ► The electrode chemically binds oxidation mediator and glucoseoxidase. ► An online FIA system is formed based on the electrode for real-time glucose analysis. ► Glucose is produced from immobilized enz...
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| Published in: | Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2012-02, Vol.666, p.32-41 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | eng |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | [Display omitted]
► A novel gold particle modified glucose sensor carbon electrode is fabricated. ► The electrode chemically binds oxidation mediator and glucoseoxidase. ► An online FIA system is formed based on the electrode for real-time glucose analysis. ► Glucose is produced from immobilized enzyme hydrolysis of waste bamboo chopsticks. ► The analysis results are comparable to that of online HPLC-RI system.
Dual on-line flow injection analysis (FIA) systems were coupled to an immobilized enzyme reactor. The primary system was detected by amperometric enzyme electrode through a specifically designed three-electrode flow cell and a stopped-flow sampling and injection device to perform the successive real-time determination of glucose in the enzyme hydrolysate of waste bamboo chopsticks fibers. The secondary system was established via high-performance liquid chromatography-refractive index detection for comparison purpose. The novel enzyme electrode was made by chemically bonding both the redox mediator ferrocenecarboxaldehyde and glucose oxidase to a gold-particles modified pencil lead carbon electrode that possessed the widest linear standard calibration concentration range of 0–39.0
mM (
r
2
=
0.9956), a limit of detection of 7.8
μM, fast response time of 5
s, and a detection sensitivity of 2212
nA
mM
−1. The electrode was usable more than 50
days. The greatest amount of glucose produced from hydrolysis was 0.46
mM at 32
h. Interference from mannose and galactose was 7% and 5%, respectively, that was neglected by their small concentration in hydrolysate. The on-line FIA of amperometric enzyme electrode detection has good analytical precision (RSD 2.2–4.5%) and accuracy (94.4–97.7%) for the real-time glucose determination. The statistical analysis shows that no systematic error exists between the quantitative results of two FIA systems. |
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| ISSN: | 1572-6657 1873-2569 |