Enhanced hydrogen production and biological saccharification from spent mushroom compost by Clostridium thermocellum 27405 supplemented with recombinant β-glucosidases
Clostridium thermocellum 27405 is a potential consolidated bioprocessing (CBP) strain due to the efficient lignocellulose degradation ability. Here, the spent mushroom substrate (SMS), a lignocellulosic byproduct of agriculture, was used as a substrate for hydrogen production by C. thermocellum. SMS...
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| Published in: | International journal of hydrogen energy 2017-03, Vol.42 (12), p.7866-7874 |
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| Main Authors: | , |
| Format: | Article |
| Language: | eng |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Clostridium thermocellum 27405 is a potential consolidated bioprocessing (CBP) strain due to the efficient lignocellulose degradation ability. Here, the spent mushroom substrate (SMS), a lignocellulosic byproduct of agriculture, was used as a substrate for hydrogen production by C. thermocellum. SMS showed a good performance for hydrogen production and the hydrogen production was significantly enhanced by C. thermocellum supplemented with β-glucosidases, with the highest production reaching 56.0 mM, an increase of 37%. Additionally, β-glucosidase and Triton X-100 had a synergistic effect on biological saccharification, resulted in an accumulation of 5.06 g/L reducing sugars, an increase of 28%. However, fusion β-glucosidase showed no improvement on hydrogen production and biological saccharification. The present study demonstrates the different influences of β-glucosidase and fusion β-glucosidase on hydrogen production and biological saccharification in CBP system and provides valuable information on the biodegradation of SMS using C. thermocellum.
•β-glucosidase significantly enhanced hydrogen production.•β-glucosidase and Triton X-100 had a synergistic effect on biosaccharification.•β-glucosidase and Triton X-100 provided a balanced way for bio-refinery.•Fusion β-glucosidase showed no beneficial effect on the biodegradation of SMS. |
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| ISSN: | 0360-3199 1879-3487 |