The effect of a period of intensive exercise on the isoform test to detect growth hormone doping in sports

Abstract Objective The major objective of this study was to investigate the effects of several days of intense exercise on growth hormone (hGH) testing using the World Anti-Doping Agencies hGH isoform differential immunoassays. Additionally the effects of circadian variation and exercise type on the...

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Bibliographic Details
Published in:	Growth hormone & IGF research 2013-08, Vol.23 (4), p.105-108
Main Authors:	Voss, S.C, Giraud, S, Alsayrafi, M, Bourdon, P.C, Schumacher, Y.O, Saugy, M, Robinson, N
Format:	Article
Language:	eng
Subjects:	Adult Advanced Basic Science Doping Doping in Sports Endocrinology & Metabolism Exercise Exercise - physiology hGH Human Growth Hormone - administration & dosage Human Growth Hormone - blood Humans Immunoassay Isoforms Male Protein Isoforms Sport Substance Abuse Detection - methods Time Factors
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Summary:	Abstract Objective The major objective of this study was to investigate the effects of several days of intense exercise on growth hormone (hGH) testing using the World Anti-Doping Agencies hGH isoform differential immunoassays. Additionally the effects of circadian variation and exercise type on the isoform ratios were also investigated. Study design 15 male athletes performed a simulated nine day cycling stage race. Blood samples were collected twice daily over a period of 15 days (stage race + three days before and after). hGH isoforms were analysed by the official WADA immunoassays (CMZ Assay GmbH). Results All measured isoform ratios were far below the WADA decision limits for an adverse analytical finding. Changes in the isoform ratios could not be clearly connected to circadian variation, exercise duration or intensity. Conclusions The present study demonstrates that the hGH isoform ratios are not significantly affected by exercise or circadian variation. We demonstrated that heavy, long term exercise does not interfere with the decision limits for an adverse analytical finding.
ISSN:	1096-6374 1532-2238