Engineering cyanobacteria with enhanced growth in simulated flue gases for high-yield bioethanol production

Engineering cyanobacteria with enhanced growth in simulated flue gases for high-yield bioethanol production

•Co-expression of ictB and ecaA in PCC7942 leads to a higher growth rate under 25% CO2.•Co-expression of ictB, ecaA, and groESL in ET6 strain increases ethanol production from flue gas.•The proposed approach improves flue gas CO2 utilization for enhanced ethanol production. Flue gases emitted from i...

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Bibliographic Details
Published in:Biochemical engineering journal 2021-01, Vol.165, p.107823, Article 107823
Main Authors: Chou, Hsiang-Hui, Su, Hsiang-Yen, Chow, Te-Jin, Lee, Tse-Min, Cheng, Wen-Hsi, Chang, Jo-Shu, Chen, Hsien-Jung
Format: Article
Language:eng
Subjects:
Bioethanol
ecaA
Flue gas
ictB
Recombinant cyanobacteria
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Summary:•Co-expression of ictB and ecaA in PCC7942 leads to a higher growth rate under 25% CO2.•Co-expression of ictB, ecaA, and groESL in ET6 strain increases ethanol production from flue gas.•The proposed approach improves flue gas CO2 utilization for enhanced ethanol production. Flue gases emitted from industrial plants mainly contribute to global CO2 emissions. Sequestrations of CO2 from the flue gas could reduce the impact of CO2 on global warming. In this study, recombinant cyanobacterial strains with enhanced photosynthetic activity, cell growth, and ethanol production were generated by co-overexpressing ictB, ecaA, and groESL, along with a heterologous ethanol synthesis pathway (pdc-adhII genes from Zymomonas mobilis) in S. elongatus PCC7942. The engineered S. elongatus PCC7942 exhibited a significant improvement in cell growth and ethanol production under a simulated flue gas consisted of 25% CO2, 80−90 ppm SO2, 90−100 ppm NO. The present work represents the first attempt of direct photoconversion of CO2 from flue gases to ethanol via expression of ictB, ecaA, groESL, and pdc-adhII in S. elongatus. The transgenic cyanobacterium becomes useful for sequestrations of CO2 directly from flue gases with the simultaneous production of bioethanol.
ISSN:1369-703X
1873-295X