Molecular markers detect redundancy and miss-identity in genetic stocks with alien leaf rust resistance genes Lr32 and Lr28 in bread wheat

Ten elite near-isogenic line (NIL) pairs of bread wheat (Triticum aestivum L em Thell) each carrying one of the two alien leaf rust resistance (Lr) genes Lr32 and Lr28, derived from Triticum tauschii and Triticum speltoides, respectively were tested for disease phenotype in controlled conditions. Th...

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Published in:Journal of plant biochemistry and biotechnology 2003-07, Vol.12 (2), p.123-129
Main Authors: Prabhu, K.V, Gupta, S.K, Charpe, A, Koul, S, Cherukuri, D.P. (Indian Agricultural Research Institute, New Delhi (India). National Phytotron Facility), Dhaliwal, H.S, Vikal, Y, Chhuneja, P. (Punjab Agricultural University, Ludhiana (India). Department of Genetics and Biotechnology), Haq, Q.M.R. (Jamia Millia Islamia, New Delhi (India). Department of Biosciences)
Format: Article
Language:English
Subjects:
clones
disease resistance
genes
genetic markers
genetic polymorphism
microsatellites
pcr
phenotypes
plant breeding
puccinia recondita
rapd
soft wheat
triticum
varieties
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Summary:Ten elite near-isogenic line (NIL) pairs of bread wheat (Triticum aestivum L em Thell) each carrying one of the two alien leaf rust resistance (Lr) genes Lr32 and Lr28, derived from Triticum tauschii and Triticum speltoides, respectively were tested for disease phenotype in controlled conditions. The disease phenotype of the NIL pair detected distinction between the Lr32 donor parent and tis derivatives in ten cultivar backgrounds documented as carrying the gene Lr32. The RAPD and SCAR molecular markers identified earlier as linked to Lr32 amplified the critical marker bands identically in eight of the ten NIL pairs as well as the Lr28 donor parent. The critical bands were not amplified in the Lr32 donor parent. A Triticum speltoides specific microsatellite null allele marker located on chromosome 4AL, the genomic region associated with Lr28, expressed in an identical polymorphism as the RAPD and SCAR markers. The PCR product sequenced from a NIL pari revealed 100% homology. It is confirmed that eight of the ten elite Lr32 lines carry the gene Lr28. Molecular marker tools need to he employed to eliminate such miss-identities and reduce redundancy in Indian elite germplasm stocks of wheat possessing the alien Lr genes.
ISSN:0971-7811
0974-1275
DOI:10.1007/BF03263172