Modulation of lymphocyte activating factor activity (interleukin 1 like activity) and acute phase proteins in pertussis-induced air pouch inflammation by muramyl dipeptide

We have studied the effect of the macrophage activator, muramyl dipeptide (MDP) on immune inflammation induced in the rat six day subcutaneous air pouch. Treated animals received either 100 micrograms or 200 micrograms MDP at the time of challenge and twenty four hours before exudate harvest. Using...

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Bibliographic Details
Published in:Agents and Actions 1987-06, Vol.21 (1-2), p.113-120
Main Authors: Sedgwick, A D, Hooke, R E, Lees, P, May, S A
Format: Article
Language:English
Subjects:
Acetylmuramyl-Alanyl-Isoglutamine - pharmacology
Animals
Connective Tissue - pathology
Dinoprostone
Disease Models, Animal - pathology
Exudates and Transudates - analysis
Inflammation - chemically induced
Inflammation - metabolism
Injections, Subcutaneous
Interleukin-1 - analysis
Interleukin-1 - antagonists & inhibitors
Leukocyte Count - drug effects
Macrophage Activation - drug effects
Male
Orosomucoid - analysis
Pertussis Vaccine - toxicity
Prostaglandins E - biosynthesis
Rats
Synovitis - pathology
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Summary:We have studied the effect of the macrophage activator, muramyl dipeptide (MDP) on immune inflammation induced in the rat six day subcutaneous air pouch. Treated animals received either 100 micrograms or 200 micrograms MDP at the time of challenge and twenty four hours before exudate harvest. Using the thymocyte co-mitogenic assay for lymphocyte activating factor (LAF), 100 micrograms MDP enhanced LAF activity whereas 200 micrograms caused inhibition. Increased dilution of 200 micrograms exudate in this assay removed this inhibition. Similarly, at the lower dose, MDP caused enhanced production of the acute phase protein alpha 1 glycoprotein, whereas the higher dose had no effect. The present study suggests that macrophage activity can be manipulated in vivo to produce LAF and naturally occurring inhibitors of LAF. These studies indicate that the stimulation of LAF inhibitors by MDP may be a potential therapeutic action.
ISSN:0065-4299
1420-908X
DOI:10.1007/BF01974931