Catabolin/interleukin-1 regulation of cartilage and chondrocyte metabolism

Catabolin/interleukin-1 effects on metabolism were studied in bovine nasal cartilage organ culture and articular chondrocyte cell culture. Keratan sulfate (KS) and hyaluronic acid (HA) were determined by an ELISA; prostaglandin E2 by RIA, sulfated glycosaminoglycan using dimethylmethylene blue and p...

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Bibliographic Details
Published in:Agents and Actions 1987-08, Vol.21 (3-4), p.341-344
Main Authors: O'Byrne, E M, Schroder, H C, Stefano, C, Goldberg, R L
Format: Article
Language:English
Subjects:
Animals
Cartilage - drug effects
Cartilage - metabolism
Cattle
Cell Division - drug effects
Culture Techniques
Dinoprostone
Hyaluronic Acid - biosynthesis
Interleukin-1 - pharmacology
Interleukin-1beta
Keratan Sulfate - metabolism
Prostaglandins E - biosynthesis
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Summary:Catabolin/interleukin-1 effects on metabolism were studied in bovine nasal cartilage organ culture and articular chondrocyte cell culture. Keratan sulfate (KS) and hyaluronic acid (HA) were determined by an ELISA; prostaglandin E2 by RIA, sulfated glycosaminoglycan using dimethylmethylene blue and proliferation by incorporation of tritiated thymidine. Gel filtration of untreated 4-day organ culture media indicated that large sulfated and KS-containing proteoglycans were released and eluted in the void volume. Catabolin/interleukin-1 increased release of sulfated glycosaminoglycans and these were of lower molecular weight with an altered distribution of KS. Catabolin/interleukin-1 treatment of chondrocytes caused a decrease in KS production and proliferation but an increase in HA and in prostaglanding E2 production. Alterations of the chondrocyte metabolism by catabolin/interleukin-1 causing proteoglycan matrix degradation and modulation of chondrocyte glycosaminoglycan biosynthesis and proliferation may play a role in cartilage erosion and failure to repair in arthritic diseases.
ISSN:0065-4299
1420-908X
DOI:10.1007/BF01966510