The effect of circulating antigen on radioimmunodetection and monoclonal antibody localisation: studies in a normal rat model

The effect of circulating antigen (human kappa light chains (LC] on radioimmunoscintigraphy was examined in a normal rat model. Rats were implanted subcutaneously with kappa (test) and lambda (control) LC coupled to sepharose beads (500 micrograms LC/ml swollen gel). When 131I-labelled monoclonal an...

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Bibliographic Details
Published in:European Journal of Nuclear Medicine 1989-06, Vol.15 (6), p.313-320
Main Authors: MCKAY, D. R, BAUTOVICH, G. J, WILSON, M. R, WALKER, K. Z
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Biological and medical sciences
Female
Humans
Immunoglobulin kappa-Chains
Immunoglobulin lambda-Chains
Investigative techniques, diagnostic techniques (general aspects)
Iodine Radioisotopes
Male
Medical sciences
Radiodiagnosis. Nmr imagery. Nmr spectrometry
Radionuclide Imaging - methods
Rats
Rats, Inbred F344
Tissue Distribution
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Summary:The effect of circulating antigen (human kappa light chains (LC] on radioimmunoscintigraphy was examined in a normal rat model. Rats were implanted subcutaneously with kappa (test) and lambda (control) LC coupled to sepharose beads (500 micrograms LC/ml swollen gel). When 131I-labelled monoclonal antibody, K-1-21 was injected, clear images of specific antibody localisation to the kappa implant were seen 6 days later. The kappa implant: blood ratio (K:B) was 7.69 +/- 0.45, which represented an implant uptake of 2.52 +/- 0.20 percent injected dose per gram (%ID/g). When 131I-K-1-21 was preincubated with 125I-kappa LC at ratios of up to 1:10 antibody: antigen, (w/w) before injection; or if antigen was given daily by bolus injection or continuously infused via an osmotic pump, very clear specific images of antibody localisation to the kappa implant were still obtained. There was, however, a decline in the absolute uptake of label by the kappa implant. The greatest change was observed following continuous antigen infusion, when kappa implant uptake fell to 1.26 +/- 0.1 %ID/g. Nevertheless, the K:B ratio was still maintained at 6.8 +/- 0.2, due to increased clearance of antibody from the blood. Thus, in this case, where antigen and antibody might be expected to form small, soluble immune complexes, there was no major deleterious effect on radioimmunoscintigraphy.
ISSN:0340-6997
1619-7089
DOI:10.1007/BF00435472