Cellulase-poor xylanases produced by Trichoderma reesei RUT C-30 on hemicellulose substrates

Hemicellulose components from industrial viscose fibre production are characterized by a lower cellulose content than commercial xylan and the presence of a carboxylic acid fraction originating from the alkaline degradation of carbohydrates during the process. This substrate, after neutralization, c...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1992-12, Vol.38 (3), p.315-322
Main Authors: Gamerith, G, Groicher, R, Zeilinger, S, Herzog, P, Kubicek, C.P
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Biotechnology & Applied Microbiology
bleaching
carboxylic acids
cellobiose
cellulases
enzyme activity
enzyme bleaching
Enzyme engineering
Fagus
Fermentation
Fundamental and applied biological sciences. Psychology
growth
hemicellulose
hydrolases
Hypocrea jecorina
inhibition
Life Sciences & Biomedicine
Methods. Procedures. Technologies
organic wastes
Production of selected enzymes
Science & Technology
sulfite pulps
Trichoderma reesei
wood pulp
xylan
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Summary:Hemicellulose components from industrial viscose fibre production are characterized by a lower cellulose content than commercial xylan and the presence of a carboxylic acid fraction originating from the alkaline degradation of carbohydrates during the process. This substrate, after neutralization, can be used by Trichoderma reesei RUT C-30 for the production of cellulase-poor xylanases, useful for the pulp and paper industry. The yields of xylanase ranged up to almost 400 units/ml, with a ratio of carboxymethylcellulase/xylanase of less than 0.015. This crude xylanase enzyme mixture was shown to be superior to that obtained on beech-wood xylan when used for bleaching and, particularly, upgrading of hard-wood chemical pulp by selective removal of the xylan components. Biochemical studies indicate that the low cellulase production by T. reesei grown on these waste hemicelluloses is the result of a combination of at least three factors: (a) the comparatively low content of cellulose in these hemicellulosic wastes, (b) the inhibitory action of the carboxylic acid fraction present in the hemicellulosic wastes on growth and sporulation of T. reesei, and (c) the use of a mycelial inoculum that is unable to initiate the attack on the cellulose components within the carbon source.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00170079