1α,25-DIHYDROXYVITAMIN D3 (CALCITRIOL) INDUCES APOPTOSIS IN STIMULATED T CELLS THROUGH AN IL-2 DEPENDENT MECHANISM

The steroid hormone 1α,25-dihydroxyvitamin D3 (calcitriol) has been shown to inhibit PHA-induced T-cell proliferation. In the present work we show that calcitriol caused a concentration-dependent decrease in the viability of` PHA stimulated but not unstimulated T lymphocytes. Under microscopy, nonvi...

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Bibliographic Details
Published in:Cytokine (Philadelphia, Pa.) Pa.), 1996, Vol.8 (5), p.342-345
Main Authors: Pintado, C.O., Carracedo, J., Rodrı&#x0301, guez, M., Pérez-Calderón, R., Ramı&#x0301, rez, R.
Format: Article
Language:English
Subjects:
1 α, 25 dihydroxyvitamin D3
apoptosis
Calcitriol
IL-2
T cells
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Summary:The steroid hormone 1α,25-dihydroxyvitamin D3 (calcitriol) has been shown to inhibit PHA-induced T-cell proliferation. In the present work we show that calcitriol caused a concentration-dependent decrease in the viability of` PHA stimulated but not unstimulated T lymphocytes. Under microscopy, nonviable cells exhibit classic features of programmed cell death or apoptosis. In addition, the same concentration of calcitriol induces a blockage in the G1 phase of T cell cycle and decreased secretion of IL-2, which was correlated with the extent of apoptosis. Thus, addition of exogenous IL-2 reverted both the cell cycle block and the apoptotic cell death induced by calcitriol. This data indicates that calcitriol induced apoptotis in PHA-stimulated T cell, and suggests that this action is mediated through suppression of IL-2 secretion.
ISSN:1043-4666
1096-0023
DOI:10.1006/cyto.1996.0047