α6 Integrin Is the Main Receptor of Human Papillomavirus Type 16 VLP

The present study was performed to determine the specific receptor of type HPV-16 using recombinant human papillomavirus-like particle (HPV-16 L1-VLP). The expression levels of α6, β1, and β4 integrins were determined and compared with the amount of HPV-VLP binding in ten cell lines by flow cytometr...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-05, Vol.283 (3), p.668-673
Main Authors: Yoon, Chun-Sik, Kim, Kwang-Dong, Park, Sue-Nie, Cheong, Seon-Woo
Format: Article
Language:English
Subjects:
HPV-16
integrin
papillomavirus
receptor
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The present study was performed to determine the specific receptor of type HPV-16 using recombinant human papillomavirus-like particle (HPV-16 L1-VLP). The expression levels of α6, β1, and β4 integrins were determined and compared with the amount of HPV-VLP binding in ten cell lines by flow cytometry. Our results show that the amount of VLP binding and the expression level of α6 integrin are correlated, which was confirmed by an inhibition experiment using antibodies and by immunocytochemistry. Both the expression level of α6 integrin and the amount of HPV-VLP binding were high in cervical cancer cell lines, as the type HPV-16 is the main cause of cervical cancer. The degree of binding of HPV-VLP matched the α6 integrin expression level in cell lines but was not correlated with β1 and β4 levels, which suggests that α6 integrin is the main receptor of HPV type 16.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.4838