DJ-1, a Novel Oncogene Which Transforms Mouse NIH3T3 Cells in Cooperation withras

We have isolated and characterized the cDNA encoding a novel protein designated DJ-1. DJ-1, sharing no significant homology with the sequences so far reported, did not show transactivation activity in the Gal4 recombinant system, but transformed mouse NIH3T3 cells by itself. Furthermore, DJ-1 showed...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1997-02, Vol.231 (2), p.509-513
Main Authors: Nagakubo, Daisuke, Taira, Takahiro, Kitaura, Hirotake, Ikeda, Masako, Tamai, Katsuyuki, Iguchi-Ariga, Sanae M.M., Ariga, Hiroyoshi
Format: Article
Language:English
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Summary:We have isolated and characterized the cDNA encoding a novel protein designated DJ-1. DJ-1, sharing no significant homology with the sequences so far reported, did not show transactivation activity in the Gal4 recombinant system, but transformed mouse NIH3T3 cells by itself. Furthermore, DJ-1 showed a cooperative transforming activity with H-Ras, more than 3 times as strong as the activity ofras/myccombination. DJ-1 was ubiquitously expressed in various human tissues, and the expression was induced by growth stimuli. Moreover, DJ-1 translocated from cytoplasm to nuclei in the S phase of the cell cycle. DJ-1 is thus suggested to be a novel mitogen-dependent oncogene product involved in a Ras-related signal transduction pathway.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1997.6132