Bioelectrochemical Analysis of Neuropathy Target Esterase Activity in Blood

Bioelectrochemical analysis of neuropathy target esterase (NTE) and its inhibitors is based on the combination of the NTE-catalyzed hydrolysis of phenyl valerate and phenol detection by a tyrosinase carbon-paste electrode. The use of the tyrosinase electrode improves 10-fold the sensitivity of NTE d...

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Bibliographic Details
Published in:Analytical biochemistry 2001-03, Vol.290 (1), p.1-9
Main Authors: Sigolaeva, Larisa V., Makower, Alexander, Eremenko, Arkadi V., Makhaeva, Galina F., Malygin, Vladimir V., Kurochkin, Ilya N., Scheller, Frieder W.
Format: Article
Language:English
Subjects:
Animals
Biosensing Techniques
blood
Brain - enzymology
Carboxylic Ester Hydrolases - antagonists & inhibitors
Carboxylic Ester Hydrolases - blood
di-2-propyl phosphorofluoridate (DFP)
Electrochemistry - methods
Enzyme Activation - drug effects
Enzyme Inhibitors - pharmacology
Humans
lymphocytes
Lymphocytes - metabolism
mipafox
Monophenol Monooxygenase - antagonists & inhibitors
Monophenol Monooxygenase - metabolism
neuropathy target esterase (NTE)
Organophosphates - toxicity
organophosphorus compound (OP)
Paraoxon - pharmacology
phenolic biosensor
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry
tyrosinase
Valerates - metabolism
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Summary:Bioelectrochemical analysis of neuropathy target esterase (NTE) and its inhibitors is based on the combination of the NTE-catalyzed hydrolysis of phenyl valerate and phenol detection by a tyrosinase carbon-paste electrode. The use of the tyrosinase electrode improves 10-fold the sensitivity of NTE detection in comparison with a spectrophotometric method. The tyrosinase electrode was found to be suitable for measurements in whole human blood where spectrophotometric detection is considerably restricted. The specificity of NTE in blood for mipafox and di-2-propyl phosphorofluoridate was close to that for neuronal NTE. The NTE-like activity in blood was determined to be 0.19 ± 0.02 nmol/min/mg of protein.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.2000.4822