Effects of nonylphenol ethoxylate exposure on reproductive output and bioindicators of environmental estrogen exposure in fathead minnows, Pimephales promelas

Nonylphenol ethoxylates (NPEOs) were evaluated in the laboratory for potential effects on the reproductive physiology and fecundity of fathead minnows (Pimephales promelas). Groups of three adult male and three female fathead minnows were exposed in a continuous flow‐through system to 0, 0.21, 0.65,...

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Bibliographic Details
Published in:Environmental toxicology and chemistry 2001-03, Vol.20 (3), p.510-522
Main Authors: Nichols, Krista M., Snyder, Erin M., Snyder, Shane A., Pierens, Susan L., Miles-Richardson, Stephanie R., Giesy, John P.
Format: Article
Language:English
Subjects:
Agnatha. Pisces
Animal, plant and microbial ecology
Animals
Applied ecology
Biological and medical sciences
Biomarkers - blood
Cyprinidae - physiology
Detergents - pharmacology
Detergents - toxicity
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on vertebrates
Endocrine
Environmental Exposure
Enzyme-Linked Immunosorbent Assay - methods
Estradiol
Estradiol - blood
Estrogens - pharmacology
Estrogens - toxicity
Ethylene Glycols - pharmacology
Ethylene Glycols - toxicity
Female
Fertility - drug effects
Fundamental and applied biological sciences. Psychology
Goldfish - physiology
Hormones
Male
Reproducibility of Results
Reproduction
Reproduction - drug effects
Sex Factors
Testosterone - blood
Vitellogenin
Vitellogenins - biosynthesis
Vitellogenins - blood
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Summary:Nonylphenol ethoxylates (NPEOs) were evaluated in the laboratory for potential effects on the reproductive physiology and fecundity of fathead minnows (Pimephales promelas). Groups of three adult male and three female fathead minnows were exposed in a continuous flow‐through system to 0, 0.21, 0.65, 2.1, or 7.9 μg NPEO/L for 42 d. Rabbit anti‐goldfish vitellogenin (VTG) antiserum was prepared and a competitive enzyme‐linked immunosorbent assay (ELISA) was adapted for measurement of plasma VTG in fish following exposure. Plasma 17β‐estradiol (E2) and testosterone (T) were also quantified by ELISA at the end of the exposure. Neither survival nor fecundity of fathead minnows exhibited a concentration‐dependent response to NPEOs. No significant differences were observed in plasma VTG concentrations among treatments for males or females. Mean plasma VTG concentrations in females ranged from 291.7 to 895.1 μ VTG/ml among treatments and did not overlap with mean concentrations measured in the plasma of males, which ranged from less than the method detection limit (0.27 μg VTG/ml) to 3.2 μg VTG/ml. Plasma E2 concentrations exhibited a significant difference between males and females within all NPEO treatments, but no differences were observed among treatments. Similarly, plasma T concentrations did not exhibit a concentration‐dependent response to NPEOs.
ISSN:0730-7268
1552-8618
DOI:10.1002/etc.5620200309