Peroxynitrite/Nitric Oxide Balance in Ischemia/Reperfusion Injury-Nanomedical Approach

Electrochemical nanosensors were used to simultaneously monitor in vitro (a single endothelial cell) and in vivo (vasculature of rat) the concentrations of NO (vasorelaxant), cytotoxic O$\rm{ {_{2}^{-}}}$ (oxidative stress) and ONOO− (nitroxidative stress). A balance of [NO]/[ONOO−]=(K) was applied...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2006-02, Vol.18 (4), p.410-416
Main Authors: Kubant, Ruslan, Malinski, Christopher, Burewicz, Andrzej, Malinski, Tadeusz
Format: Article
Language:English
Subjects:
Amperometric detection
In situ
Modified electrodes
Nitric oxide
Superoxide
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Summary:Electrochemical nanosensors were used to simultaneously monitor in vitro (a single endothelial cell) and in vivo (vasculature of rat) the concentrations of NO (vasorelaxant), cytotoxic O$\rm{ {_{2}^{-}}}$ (oxidative stress) and ONOO− (nitroxidative stress). A balance of [NO]/[ONOO−]=(K) was applied as the diagnostic marker of dysfunctional endothelium and cardiovascular disease. In the isolated endothelium of normotensive rats, K= 2.8±0.1 while in hypertensive rats, K=0.4±0.1. During ischemia, K dropped from 7±1 to 1.4±0.2 and further decreased to 0.05±0.01 during reperfusion. The edema and vasoconstriction, indicators of vascular injury, correlated directly with the decrease in K.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.200503436