Ribonomic Approaches to Identify Protein– mRNA and micro RNA – mRNA Interactions: Implications for Drug Design

Abstract Preclinical Research Posttranscriptional regulation of gene expression is now recognized to play a large role in physiological and pathological processes. Through interactions with messenger ribonucleic acid ( mRNA ), RNA ‐binding proteins ( RBP s) and micro RNA s ( miRNA s) can alter mRNA...

Full description

Saved in:
Bibliographic Details
Published in:Drug development research 2012-11, Vol.73 (7), p.406-413
Main Authors: Dziublenski, Matthew, Roff, Alanna N., Ishmael, Faoud T.
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Preclinical Research Posttranscriptional regulation of gene expression is now recognized to play a large role in physiological and pathological processes. Through interactions with messenger ribonucleic acid ( mRNA ), RNA ‐binding proteins ( RBP s) and micro RNA s ( miRNA s) can alter mRNA stability or translation. Aberrant function of these pathways may play a role in inflammatory diseases and cancer, making these attractive therapeutic targets. miRNA s and some RBP s, such as tristetraprolin ( TTP ), destabilize mRNA and may inhibit translation. Other RBP s, such as HuR , may increase mRNA stability to upregulate gene expression. Each RBP or a single miRNA has the potential to regulate multiple functionally related targets by binding to specific sequences or motifs on mRNA . Identification of these regulatory interactions has become a key step in understanding how gene expression is controlled and in designing drugs to target these interactions in disease. A number of ribonomic techniques have been developed to accurately identify RBP – mRNA and miRNA – mRNA binding interactions, including immunoprecipitation and affinity purification techniques combined with microarray technology and high‐throughput deoxyribonucleic acid (DNA) sequencing. Information gained from these approaches can be used to design agents that mimic or inhibit these regulatory interactions and thus represents a novel strategy for the treatment of disease.
ISSN:0272-4391
1098-2299
DOI:10.1002/ddr.21031