Optimization and Performance of Silica-Based Media for Industrial-Scale Antibody Purification

A silica‐based medium with optimized pore size, pore volume, and coupling density was prepared for protein A affinity chromatography, to be used for industrial purification of IgG. Performance and durability of the silica‐based medium were studied during repeated use and compared with other protein...

Full description

Saved in:
Bibliographic Details
Published in:Chemical engineering & technology 2012-01, Vol.35 (1), p.157-160
Main Authors: Miyahara, H., Nakashima, R., Inoue, M., Katsuda, T., Yamaji, H., Katoh, S.
Format: Article
Language:English
Subjects:
Affinity chromatography
Antibody purification
Dynamic binding capacity
Protein A
Silica medium
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A silica‐based medium with optimized pore size, pore volume, and coupling density was prepared for protein A affinity chromatography, to be used for industrial purification of IgG. Performance and durability of the silica‐based medium were studied during repeated use and compared with other protein A media available on the market. The silica‐based medium provided high dynamic binding capacities for human polyclonal IgG in the superficial liquid velocity range from 150 to 720 cm h–1 because of a high mass transfer coefficient and high mechanical strength. The dynamic binding capacity and IgG recovery were high and varied only slightly during 50–150 cycles of repeated use. The release of protein A ligand was low. A silica‐based medium with optimized pore size, pore volume, and coupling density was developed for protein A affinity chromatography, to be used for industrial purification of IgG. Compared with common protein A media, it provided a high mass transfer rate and high dynamic binding capacities for IgG.
ISSN:0930-7516
1521-4125
DOI:10.1002/ceat.201100245