FIONA1-Mediated m 6 A Modification Regulates the Floral Transition in Arabidopsis

N -methyladenosine (m A) mRNA modification represents the most widespread form of internal modifications in eukaryotic mRNAs. In the model plant Arabidopsis thaliana, those known methyltransferases mainly deposit m A at their target transcripts near the stop codon or in the 3' untranslated regi...

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Published in:Advanced science 2022-02, Vol.9 (6), p.e2103628
Main Authors: Xu, Tao, Wu, Xiaowei, Wong, Chui Eng, Fan, Sheng, Zhang, Yu, Zhang, Songyao, Liang, Zhe, Yu, Hao, Shen, Lisha
Format: Article
Language:English
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Summary:N -methyladenosine (m A) mRNA modification represents the most widespread form of internal modifications in eukaryotic mRNAs. In the model plant Arabidopsis thaliana, those known methyltransferases mainly deposit m A at their target transcripts near the stop codon or in the 3' untranslated region. Here, it is reported that FIONA1 (FIO1), a human METTL16 ortholog, acts as a hitherto unknown m A methyltransferase that determines m A modifications at over 2000 Arabidopsis transcripts predominantly in the coding region. Mutants of FIO1 show a decrease in global m A mRNA methylation levels and an early-flowering phenotype. Nanopore direct RNA sequencing reveals that FIO1 is required for establishing appropriate levels of m A preferentially in the coding sequences of a subset of protein-coding transcripts, which is associated with changes in transcript abundance and alternative polyadenylation. It is further demonstrated that FIO1-mediated m A methylation determines the mRNA abundance of a central flowering integrator SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and its upstream regulators, thus preventing premature flowering. The findings reveal that FIO1 acts as a unique m A methyltransferase that mainly modifies the coding regions of transcripts, which underlies the key developmental transition from vegetative to reproductive growth in plants.
ISSN:2198-3844
2198-3844
DOI:10.1002/advs.202103628