Engineered Bacterial Bioreactor for Tumor Therapy via Fenton-Like Reaction with Localized H 2 O 2 Generation

Synthetic biology based on bacteria has been displayed in antitumor therapy and shown good performance. In this study, an engineered bacterium Escherichia coli MG1655 is designed with NDH-2 enzyme (respiratory chain enzyme II) overexpression (Ec-pE), which can colonize in tumor regions and increase...

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Bibliographic Details
Published in:Advanced materials (Weinheim) 2019-04, Vol.31 (16), p.e1808278
Main Authors: Fan, Jin-Xuan, Peng, Meng-Yun, Wang, He, Zheng, Hao-Ran, Liu, Zong-Lin, Li, Chu-Xin, Wang, Xia-Nan, Liu, Xin-Hua, Cheng, Si-Xue, Zhang, Xian-Zheng
Format: Article
Language:English
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Summary:Synthetic biology based on bacteria has been displayed in antitumor therapy and shown good performance. In this study, an engineered bacterium Escherichia coli MG1655 is designed with NDH-2 enzyme (respiratory chain enzyme II) overexpression (Ec-pE), which can colonize in tumor regions and increase localized H O generation. Following from this, magnetic Fe O nanoparticles are covalently linked to bacteria to act as a catalyst for a Fenton-like reaction, which converts H O to toxic hydroxyl radicals (•OH) for tumor therapy. In this constructed bioreactor, the Fenton-like reaction occurs with sustainably synthesized H O produced by engineered bacteria, and severe tumor apoptosis is induced via the produced toxic •OH. These results show that this bioreactor can achieve effective tumor colonization, and realize a self-supplied therapeutic Fenton-like reaction without additional H O provision.
ISSN:0935-9648
1521-4095
DOI:10.1002/adma.201808278