Effect of electroacpuncture of Neiguan （PC 6） and Tianquan （PC 2） on skin temperature, blood perfusion, and adrenoceptor response in rats with acute myocardial ischemia

OBJECTIVE： To investigate the effect of electroacu-puncture （EA） of acupoints Neiguan （PC 6） and Tian-quan （PC 2） on the skin temperature, blood perfu-sion, and alpha1- and beta2-adrenoreceptor （al-ARand β2-AR） protein and mRNA level in rats withacute myocardial ischemia.METHODS： Thirty male adult W...

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Published in:中医杂志：英文版 2017, Vol.37 (6), p.794-803
Main Authors: Wang Dan, Ji Bo, Zhao Guozhen, Yan Mingna, Bai Hongxin, Sun Xiaomin, Dai Jian, Lu Yawen, Liu Yitian, You Min, Wu Jiaojuan, Ge Yunpeng, Su Hang, Cheng Kai
Format: Article
Language:English
Subjects:
Electroacupuncture
Myocardial
isch-emia
Point
PC
6
（Neiguan）,
Point
PC
2
（Tian-
quan）,
Skin
temperature
Skin
blood
perfusion,Adrenoceptor
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Summary:OBJECTIVE： To investigate the effect of electroacu-puncture （EA） of acupoints Neiguan （PC 6） and Tian-quan （PC 2） on the skin temperature, blood perfu-sion, and alpha1- and beta2-adrenoreceptor （al-ARand β2-AR） protein and mRNA level in rats withacute myocardial ischemia.METHODS： Thirty male adult Wistar rats [（230 ＋20） g] were randomly assigned into five groups（n = 6 each）： a control group, sham operationgroup, model group, model group treated with EAat low frequency （L-EA, 2 Hz, 1 mA） and modelgroup treated with EA at high frequency （H-EA, 100Hz, 1 mA）. The rat model was prepared by ligatingthe left anterior descending coronary artery. Elec-troacupuncture was performed at the left Neiguan（PC 6） for 20min daily for 3 d. After the 3rd time ofthe treatment, measurements of skin blood perfu-sion and temperature in Neiguan （PC 6） and Tian-quan （PC 2） in all groups were made by laser speck-le contrast imaging and infrared thermal image in-strument. Real-time PCR and ELISA were used tomeasure mRNA level and protein level of a~-AR and132-AR in the skin tissues of Neiguan （PC 6） and Tian-quan （PC 2）, respectively. Serum levels of cTnT andelectrocardiogram were used to identify the stateof myocardium.
ISSN:0255-2922