Improvement in Mechanical Properties and Biocompatibility ofBiosynthetic Bacterial Cellulose/Lotus Root Starch Composites

Bacterial cellulose/lotus root starch （BC/LRS） composites were prepared by cultivating Acetobacter xylinum innutrient media containing gelatinized lotus root starch. Low concentrations of gelatinized LRS had increased BC productionwith the maximum value at 6.67 g/L when 5 g/L of LRS was added in the...

Full description

Saved in:
Bibliographic Details
Published in:高分子科学：英文版 2017, Vol.35 (3), p.354-364
Main Author: Na Yin Shi-yan Chen Yi-meng Cao Hua-ping Wang Qing-kai Wu
Format: Article
Language:English
Subjects:
Bacterial
biocompatibility
Biosynthesis
Cell
cellulose
Composites
Lotus
root
starch
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bacterial cellulose/lotus root starch （BC/LRS） composites were prepared by cultivating Acetobacter xylinum innutrient media containing gelatinized lotus root starch. Low concentrations of gelatinized LRS had increased BC productionwith the maximum value at 6.67 g/L when 5 g/L of LRS was added in the culture media and the composites had thicker anddenser fibrils compared with those of BC with low concentrations of LRS （2.5 and 5 g/L）. When the concentration of LRSwas increased above 7.5 g/L, the morphology of the BC/LRS composites contained more fibril layers that were linked withLRS. The results from X-ray diffraction （XRD） demonstrated that there was no significant difference in structure betweenBC and BC/LRS composites except a slight increase in crystallinity for BC/LRS composites as the concentration of LRS waslifted up. The tensile tests were performed to display BC/LRS composites prepared with LRS concentration at 2.5 and 5 g/Lin media had the tensile strength of 54 and 60 MPa, respectively, which indicated an improvement in mechanical propertycompared to the unmodified BC （45 MPa）. Live/dead assay with chondrocytes seeded on BC/LRS composite revealed highercell viability ranging from 85% to 90% than BC. Furthermore, cell morphology with typical spindle shape was observed onthe surfaces of BC/LRS composite by confocal microscope. Through the overall results, it shows that this study has provideda guidance to prepare BC/LRS composites with better cell biocompatibility and higher mechanical strength than those of BCfor the potential use in cartilage tissue engineering.
ISSN:0256-7679
1439-6203