Combining QTL mapping and expression profile analysis to identify candidate genes of cold tolerance from Dongxiang common wild rice（Oryza rufipogon Griff.）

Rice（Oryza sativa L.）, a tropical and subtropical crop, is susceptible to low temperature stress during seedling, booting, and flowering stages, which leads to lower grain quality levels and decreasing rice yields. Cold tolerance is affected by multiple genetic factors in rice, and the complex genet...

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Bibliographic Details
Published in:农业科学学报：英文版 2016 (9), p.1933-1943
Main Author: ZHAO Jie QIN Jing-jing SONG Qian SUN Chuan-qing LIU Feng-xia
Format: Article
Language:English
Subjects:
Oryza
QTL定位
东乡
候选基因
分子标记辅助选择
普通野生稻
穗期耐冷性
表达谱分析
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Summary:Rice（Oryza sativa L.）, a tropical and subtropical crop, is susceptible to low temperature stress during seedling, booting, and flowering stages, which leads to lower grain quality levels and decreasing rice yields. Cold tolerance is affected by multiple genetic factors in rice, and the complex genetic mechanisms associated with chilling stress tolerance remain unclear. Here, we detected seven quantitative trait loci（QTLs） for cold tolerance at booting stage and identified one cold tolerant line, SIL157, in an introgression line population derived from a cross between the indica variety Guichao 2, as the recipient, and Dongxiang common wild rice, as the donor. When compared with Guichao 2, SIL157 showed a stronger cold tolerance during different growth stages. Through an integrated strategy that combined QTL-mapping with expression profile analysis, six candidate genes, which were up-regulated under chilling stress at the seedling and booting developmental stages, were studied. The results may help in understanding cold tolerance mechanisms and in using beneficial alleles from wild rice to improve the cold tolerance of rice cultivars through molecular marker-assisted selection.
ISSN:2095-3119
2352-3425