Identification of Sphaeroma terebrans via morphology and the mitochondrial cytochrome c oxidase subunit I （COI） gene

Sphaeroma terebrans, a wood-boring isopoda, is distributed worldwide in tropical and subtropical mangroves. The taxonomy of S. terebrans is usually based on morphological characteristics, with its molecular identification still poorly understood. The number of teeth on the uropodal exopod and the le...

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Bibliographic Details
Published in:动物学研究 2016, Vol.37 (5), p.307-312
Main Author: Xiu-Feng LI Chong HAN Cai-Rong ZHONG Jun-Qiu XU Jian-Rong HUANG
Format: Article
Language:Chinese
Subjects:
亚基
分子识别
基因序列
形态特征
线粒体
细胞色素c氧化酶
鉴定
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Summary:Sphaeroma terebrans, a wood-boring isopoda, is distributed worldwide in tropical and subtropical mangroves. The taxonomy of S. terebrans is usually based on morphological characteristics, with its molecular identification still poorly understood. The number of teeth on the uropodal exopod and the length of the propodus of the seventh pereopod are considered as the major morphological characteristics in S. terebrans, which can cause difficulty in regards to accurate identification. In this study, we identified S. terebrans via molecular and morphological data. Furthermore, the validity of the mitochondrial cytochrome c oxidase subunit I （COl） gene as a DNA barcode for the identification of genus Sphaeroma, including species S. terebrans, S. retrolaeve, and S. serratum, was examined. The mitochondrial COl gene sequences of all specimens were sequenced and analysed. The interspecific Kimura 2-parameter distances were higher than intraspecific distances and no intraspecificinterspecific distance overlaps were obs
ISSN:0254-5853