Effects of different Chinese herbal prescriptions on cytokines in autoimmune prostatitis rats

OBJECTIVE：To observe and compare the effects of Chinese herbal prescriptions for promoting blood circulation,clearing heat,removing toxicity,and dispersing stagnated liver-Qi on cytokines in model rats with experimental autoimmune prostatitis（EAP） to provide an experimental basis for the use of Chin...

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Published in:中医杂志：英文版 2015-04 (2), p.211-217
Main Author: Song Guohong Zhang Qiumei Pang Baozhen He Lijuan Saimaiti Julaiti Tulahong Aisikeer Gao Xuan You Lina Wuxiuer Reyihan Zhou Wentao Pang Qingyang
Format: Article
Language:English
Subjects:
dose
IL-6
IL-8
Wistar大鼠
中药方剂
慢性前列腺炎
细胞因子
自身免疫性
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Summary:OBJECTIVE：To observe and compare the effects of Chinese herbal prescriptions for promoting blood circulation,clearing heat,removing toxicity,and dispersing stagnated liver-Qi on cytokines in model rats with experimental autoimmune prostatitis（EAP） to provide an experimental basis for the use of Chinese herbal prescriptions in the treatment of chronic prostatitis.METHODS：One-hundred and ten male Wistar rats were randomly divided into 11 groups：blank group;model group;Huoxuehuayu（promoting blood circulation to remove blood stasis） high,middle,and low dose groups;Qingrejiedu（clearing heat and removing toxicity） high,middle,and low dose groups;and Shuganliqi（dispersing stagnated liver-Qi） high,middle,and low dose groups.Except the blank group,rats in all groups were injected subcutaneously in multiple points on days 0 and 30 with prostatic protein extractive solution（60 mg/ml_）,and intraperitoneally injected with diphtheria-pertussis and tetanus vaccine（DPT vaccine） to establish the EAP model.Model rats were administrated high,middle,and low doses of Chinese herbal prescriptions and were sacrificed after 4 weeks.Pathological changes in the prostate gland were observed with HE staining and changes in serum interleukin-6（IL-6）,interleukin-8（IL-8）,and prostaglandin E_2（PGE_2） levels were detected with enzyme-linked immunosorbent assay.RESULTS：Compared with the blank group,serum PGE_2,IL-6,and IL-8 levels in the model group were significantly higher（P 〈 0.05）.Compared with the model group,serum PGE_2,IL-6,and IL-8 levels in the Qingrejiedu low dose and middle dose groups were significantly lower（P 〈 0.05）,with the lower dose having a more obvious effect.Serum PGE_2,IL-6,and IL-8 levels in the Huoxuehuayu high dose group（P 〈 0.05）,IL-6 and IL-8 levels in the Huoxuehuayu middle dose group（P 〈 0.05）,and the IL-8level in the Huoxuehuayu low dose group were significantly lower than those in the model group（P 〈 0.05）.There were significant differences in PGE_2 and IL-6 levels among the different dose groups of Shuganliqi drugs（P 〈 0.05）.Compared with the model group,serum PGE_（2/） IL-6,and IL-8levels in the Shuganliqi high dose group（P 〈0.05） and IL-8 level in the Shuganliqi low dose group were significantly lower（P 〈 0.05）,while the Shuganliqi middle dose group did not change significantly（P 〉 0.05）.CONCLUSION：Therefore,in TCM treatment of autoimmune prostatitis,different treatment methods should select different doses.For prescriptions that clear heat and remove toxic
ISSN:0255-2922
DOI:10.1016/S0254-6272(15)30030-3