A DNA ligase required for active DNA demethylation anti genomic imprinting in Arabidopsis

Dear Editor, Active DNA demethylation plays crucial roles in the regulation of gene expression and gene imprinting. In plants, active DNA demethylation is initiated by the ROS1/DME family of 5-methylcytosine-specific DNA glycosylases via a base excision repair mechanism [ 1, 2]. ROS1 and DME are bif...

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Bibliographic Details
Published in:细胞研究：英文版 2015 (6), p.757-760
Main Author: Yan Li Cheng-Guo Duan Xiaohong Zhu Weiqiang Qian Jian-Kang Zhu
Format: Article
Language:English
Subjects:
DNA连接酶
去甲基化
基因组印记
拟南芥
活性
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Summary:Dear Editor, Active DNA demethylation plays crucial roles in the regulation of gene expression and gene imprinting. In plants, active DNA demethylation is initiated by the ROS1/DME family of 5-methylcytosine-specific DNA glycosylases via a base excision repair mechanism [ 1, 2]. ROS1 and DME are bifunctional DNA glycosylases that excise the 5-methylcytosine base and then cleave the DNA backbone at the abasic site, resulting in a gap with a 3＇ phosphate or 3＇ dRP （3＇ a, D-unsaturated aldehyde） terminus. The DNA phosphatase ZDP and the apurinic/ apyrimidinic endonuclease APE1L process the 3＇ phos- phate and 3＇ dRP termini, respectively, to generate a 3＇ OH group so that downstream polymerases and ligases can fill in the gap with an unmethylated cytosine [3, 4].
ISSN:1001-0602
1748-7838