Protease-activated receptor （PAR）1, PAR2 and PAR4 expressions in esophageal squamous cell carcinoma

Here, we used reverse transcription-PCR （RT-PCR） and western blot to detect protease-activated receptor （PAR） 1, PAR 2 and PAR 4 expression in cancer tissues and cell lines of esophageal squamous cell carcinoma, and investigated the co-relationship between PAR expression and clinic-pathological data...

Full description

Saved in:
Bibliographic Details
Published in:动物学研究 2014, Vol.35 (5), p.420-425
Main Author: Si-Man LI Ping JIANG Yang XIANG Wei-Wei WANG Yue-Chun ZHU Wei-Yang FENG Shu-De LI Guo-Yu YU
Format: Article
Language:Chinese
Subjects:
mRNA表达
PAR
RT-PCR
基因启动子
蛋白酶激活受体
逆转录PCR
食管癌
鳞状细胞癌
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Here, we used reverse transcription-PCR （RT-PCR） and western blot to detect protease-activated receptor （PAR） 1, PAR 2 and PAR 4 expression in cancer tissues and cell lines of esophageal squamous cell carcinoma, and investigated the co-relationship between PAR expression and clinic-pathological data for esophageal cancer. The methylation of PAR4 gene promoter involved in esophageal carcinoma was also analyzed. By comparing the mRNA expressions of normal esophageal tissue and human esophageal epithelial cells （HEEpiC）, we found that among the 28 cases of esophageal squamous cell carcinoma, PAR1 （60%） and PAR2 （71%） were elevated in 17 and 20 cases, respectively, and PAR4 （68%） expression was lowered in 19 cases. Whereas, in human esophageal squamous cells （TE-1 and TE-10）, PAR1 and PAR2 expression was increased but PAR4 was decreased. Combined with clinical data, the expression of PAR1 in poorly differentiated （P=0.016） and middle and lower parts of the esophagus （P=-0.016） was higher; expression of PAR4 in po
ISSN:0254-5853