Cloning the sterol carrier protein 2 genes of Japanese toad （Bufo japonicus formosus） and Chinese toad （Bufo gargarizans） and its tissue expression analysis

In this study, to clarify the bioactive polypeptides included in the skins and secretions of Bufo, we screened the Japanese toad （Bufo japonicus formosus） skin cDNA library by colony polymerase chain reaction （PCR）, and obtained a transcript of 1 075 bp consisting of 137 bp 5＇ untranslated region （U...

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Bibliographic Details
Published in:动物学研究 2014, Vol.35 (5), p.398-403
Main Author: Yu-Cheng JI Hui ZHUGE Shan-Shan ZHANG Shu-Fang ZHANG Xian-Yu Yang
Format: Article
Language:Chinese
Subjects:
cDNA克隆
中国
刺参
日本
组织分布
蟾蜍
表达分析
载体蛋白
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Summary:In this study, to clarify the bioactive polypeptides included in the skins and secretions of Bufo, we screened the Japanese toad （Bufo japonicus formosus） skin cDNA library by colony polymerase chain reaction （PCR）, and obtained a transcript of 1 075 bp consisting of 137 bp 5＇ untranslated region （UTR）, 515 bp 3＇ UTR and a 423 bp open reading frame （ORF） encoding a polypeptide of 140 amino acid residues （GenBank accession number： KF359945）. Homolog analysis showed a 70%-96% homology with sterol carrier protein-2 （SCP-2） present in other animals, which is implicated in lipid metabolism of other organisms. The gene SCP-2 of Chinese toad （B. gargarizans） was cloned from a first strand cDNA of Bufo skin （GenBank accession number： KF381341） via PCR, whose encoding polypeptide has only one amino acid difference from that of Japanese toad. Tissue distribution analysis showed that SCP-2 expressed in all organs tested, though in the liver and spleen it manifested lower expression than in other organs. These findings m
ISSN:0254-5853