Quantification of anandamide, oleoylethanolamide and palmitoylethanolamide in rodent brain tissue using high performance liquid chromatographyelectrospray mass spectroscopy

Reported concentrations for endocannabinoids and related lipids in biological tissues can vary greatly; therefore, methods used to quantify these compounds need to be validated. This report describes a method to quantify anandamide （AEA）, oleoylethanolamide （OEA） and palmitoylethanolamide （PEA） from...

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Bibliographic Details
Published in:药物分析学报：英文版 2014, Vol.4 (4), p.234-241
Main Author: Daniel J. Liput Eleftheria Tsakalozou Dana C. Hammell Kalpana S. Paudel Kimberly Nixon Audra L. Stinchcomb
Format: Article
Language:English
Subjects:
乙醇胺
乙醇酰胺
啮齿动物
脑组织
花生四烯酸
质谱测定
量化
高效液相
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Summary:Reported concentrations for endocannabinoids and related lipids in biological tissues can vary greatly; therefore, methods used to quantify these compounds need to be validated. This report describes a method to quantify anandamide （AEA）, oleoylethanolamide （OEA） and palmitoylethanolamide （PEA） from rodent brain tissue. Analytes were exwacted using acetonitfile without further sample clean up, resolved on a C 18 reverse-phase column using a gradient mobile phase and detected using eleclmspmy ionization in positive selected ion monitoring mode on a single quadrupole mass spectrometer. The method produced high recovery rates for AEA, OEA and PEA, ranging from 98.1% to 106.2%, 98.5% to 102.2% and 85.4% to 89.5%, respectively. The method resulted in adequate sensitivity with a lower limit of quantification for AEA, OEA
ISSN:2095-1779
2214-0883