PFA-fixed Hsp6Osp-loaded dendritic cells as a vaccine for the control of mouse experimental allergic encephalomyelitis

We have shown that Hsp6Osp-loaded immature dendritic cells （DC/sp） can protect mice from the induction of experimental allergic encephalomyelitis （EAE） by inducing Qa-l-restricted CD8＋ T regulatory （Treg） cells. The binding half-life between Qa-1 and Hsp6Osp is particularly short and leads to an uns...

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Published in:中国免疫学杂志：英文版 2014, Vol.11 (2), p.169-174
Main Author: Feng Liu Hui Zheng Yuanyuan Qi Xue Wang Jianjun Yang Miaomiao Han Hart Zhang Hong Jiang
Format: Article
Language:English
Subjects:
FDC
PFA
实验性变态反应性脑脊髓炎
小鼠
控制
树突状细胞
细胞疫苗
自身免疫性疾病
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Summary:We have shown that Hsp6Osp-loaded immature dendritic cells （DC/sp） can protect mice from the induction of experimental allergic encephalomyelitis （EAE） by inducing Qa-l-restricted CD8＋ T regulatory （Treg） cells. The binding half-life between Qa-1 and Hsp6Osp is particularly short and leads to an unstable Qa- l/peptide complex that significantly decreases the efficacy of this vaccination. To prevent Qa-l/Hsp6Osp complex dissociation, we utilized paraformaldehyde （PFA） fixation to stabilize the formation of the Qa-l/Hsp6Osp complex and maximize the function of DC/sp as a vaccine to control autoimmune diseases. Compared with the non-fixed DC/sp, the fixed DC/sp （FDC/sp） showed an enhanced ability to activate Qa-l-restricted Hsp6Osp-specific CD8＋T cells in vitro and prevented EAE in vivo. Importantly, the FDC/sp maintained immune activity following cryopreservation for I week or after storage for 72 h at 4 ~C. These results indicate that PFA fixation can sustain or increase the efficacy of DC/sp by improving the stability of the Qa-l/Hsp6Osp complex on the surface of the DC/sp. In addition, PFA fixation creates a time window for DC/sp storage, transport and application. Our d~tn ~u~p__~t ~ nnt~nti~l clinic~l ..~e nf FDCI~n ~ ~ vnccine fnr the nr~v~ntinn and treatment of autnimm.n~_ di_~_~__
ISSN:1672-7681
2042-0226